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May 19, 2022 13 tweets 10 min read Read on X
Please take your seats for @gordon_sanghera's opening plenary of London Calling 2022. #nanoporeconf
GS: Lord Kelvin believed in meaningful measurement to see the whole picture. With nanopore sequencing you gain more comprehensive insights than ever before. #nanoporeconf
GS: In the 3 years since the last time we were at Old Billingsgate, @nanopore has released 200+ products, increased our single-molecule raw read accuracy from 95% to 99.6% & hugely improved our scalability & output. #nanoporeconf
GS: This has been reflected in the productivity of the community,. >1000 publications in 2021 – 3x as many as in 2019. You are working on high throughput human genomics projects, to individual samples from answer in critical care on the same day. #nanoporeconf
GS: Nanopore sequencing is the only platform to enable native #DNA & #RNA sequencing for comprehensive real-time SNP, SV AND methylation calling within a single data set in fully scalable formats from pocket to population. #nanoporeconf
GS: Nanopore sequencing is the only platform to enable native #DNA & #RNA sequencing for comprehensive real-time SNP, SV AND methylation calling within a single data set in fully scalable formats from pocket to population. #nanoporeconf
GS: This can be highlighted by a few quotes from members of the @nanopore community. @bioinfo_mark, @danrdanny, @GershmanAriel #nanoporeconf
GS: Scalability: sequencing devices that fit the tech to the biology. #nanoporeconf
GS: We can't not mention #COVID19. Critical work in public health contributing >1 million #SARSCoV2 genomes from 85 countries. The #nanopore community made, and is still making, a significant contribution when the world needed it most. #nanoporeconf
GS: We are still at the foothills of what this technology can do. From discovery science and translational research to the potential of translation to healthcare in many communities in the future. #nanoporeconf
GS: We have seen a strong community drive for broadening surveillance applications. This includes workflows for African Swine Fever, #TB, respiratory #metagenomics & routine pathogens such as #COVID19 and influenza #nanoporeconf
GS: Our goal has always been to enable the analysis of anything by anyone, anywhere. You can check out some examples here: nanoporetech.com/portable-seque… #anythinganyoneanywhere #nanoporeconf
Gordon officially opens the first day of talks by banging the same gong we used to celebrate @nanopore being listed in the London Stock Exchange. #nanoporeconf

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More from @nanopore

Dec 31, 2022
As 2022 draws to a close, we’ve been reflecting on some of the highlights (in no particular order) — you can read the full article here: bit.ly/3IfaKYc, or digest the thread below. Wishing you a happy new year and all the best for 2023! 1/23
The #cancer research community has made huge strides leveraging the unique attributes of #nanopore sequencing to characterise cancer at unprecedented resolution, unlocking previously hidden variation and accelerating research to support human health. nanoporetech.com/cancer-research 2/23
In May, @mdelledon took a group of scientists into the Gobi Desert to capture small mammals, including bats, to study their microbiome and they set up a genomics lab in a tent. They demonstrated the utility of the MinION in the harsh, off grid and sandy desert environment! 3/23
Read 23 tweets
May 19, 2022
Time for the latest @nanopore technology updates. Up next in the auditorium: an Update from the Oxford Nanopore team. #nanoporeconf Image
Please note, we invite you to read the disclaimer in the slides
CB: More information on Clive’s previous technology updates:
Read 71 tweets
Dec 1, 2021
Opening the Nanopore Community Meeting Tech Update is James Clarke, valiantly speaking through a bad, bad cold. We'll be live tweeting in this thread, with Stu Reid and Rosemary Dokos up next #nanoporeconf
JC: @nanopore accuracy has come a long way. Right now, in just 3-4 seconds, I can pass 1,000 bases of a **single molecule** of DNA through a hole, and know its sequence with over 99% accuracy. That’s better than Q20, by looking at a single molecule - once #nanoporeconf
JC: Beyond single molecule, you can of course use multiple strands in a pile-up. We’ve achieved Q50 consensus accuracy on bacterial genomes, from 20X coverage. #nanoporeconf
Read 74 tweets
Dec 1, 2021
Up in a few minutes, Clive G Brown @the_taybor with a plenary talk on @nanopore sequencing. We'll live tweet in this thread 1/n #nanoporeconf
@The_Taybor CB: our goal is to enable the analysis of anything, by anyone, anywhere #nanoporeconf
CB: A reminder of how nanopore works #nanoporeconf
Read 42 tweets
Dec 1, 2021
For those using @ArticNetwork Midnight for #SARSCoV2 sequencing: analysis of the available data (55 genomes) showed that 100% were correctly identified as Omicron. We’ve observed 1 amplicon dropout out of 29. This is outside of the S gene & does not impact correct lineage ID 1/4
Whilst the amplicon dropout does not affect identification of the Omicron variant, the Midnight kit will be revised to include an additional primer to improve amplification efficiency of Omicron Amplicon 28. We expect this to be available in 2-4 weeks 2/4
We are again thankful to @freed_nikki and the entire @ArticNetwork community for quick and open data sharing so that we can continue to provide high quality solutions for #SARSCoV2 surveillance around the globe 3/4
Read 4 tweets
May 20, 2021
Coming up in a few minutes, Clive Brown @the_taybor, with an update entitled “Nobody Expects the Strandish Exposition”. Follow the talk live in this thread #nanoporeconf
CB: Our goal is to enable the analysis of anything by anyone, anywhere. Here are some examples of global use: nanoporetech.com/portable-seque…
CB: As a reminder, this is how nanopore sequencing works. #nanoporeconf
Read 51 tweets

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