How did we get from this first sighting to finding their identity and function? Follow this thread!
bit.ly/35o2XSj
1/n
![Discovery of mammary ductal macrophages](https://pbs.twimg.com/media/EXaarn3U4AEJSiW.jpg)
![Tissue-resident ductal macrophages survey the mammary epithelium and facilitate tissue remodelling](https://pbs.twimg.com/media/EXaa1DHU4AAuug8.jpg)
We use 3D imaging to investigate the complex relationships b/w these cells to better understand disease.
#microscopy #mammarygland
2/n
4/n
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6/n
![](https://pbs.twimg.com/media/EXabkHkU0AIhHo6.jpg)
![](https://pbs.twimg.com/media/EXabk0bU0Agp1gZ.jpg)
7/n
![Imaging of macrophage fluorescent reporter expression in the mammary gland](https://pbs.twimg.com/media/EXacWkKXkAA1zq-.jpg)
#intravital #microscopy
10/n
11/n
#microscopy
18/n
So DMs eat the dying cells, but are they necessary for remodelling? This process is like wound healing, is associated with cancer and prepares the gland for new pregnancies.
21/n
…but they are like DMs!
23/n
![](https://pbs.twimg.com/media/EXaiWOcVcAEBwZH.jpg)
![](https://pbs.twimg.com/media/EXaiW--U4AIsv79.jpg)
24/n
Please welcome DMs into the #macrophage family 🤗
Thanks to the whole, incredible team!
bit.ly/mammaryDMs bit.ly/35o2XSj
25/n
![Diagram summarizing the findings of Dawson et al in 'Tissue-resident ductal macrophages survey the mammary epithelium and facilitate tissue remodelling', published in Nature Cell Biology](https://pbs.twimg.com/media/EXajz9MUEAARKOD.jpg)
26/n
Spontaneous collaboration through #ScienceTwitter!
27/n
![](https://pbs.twimg.com/media/EXalhrGUEAA99vo.jpg)
I imaged whole embryonic glands and found one #macrophage inside each duct tip – maybe these can generate all DMs until puberty? They only turn on MHCII at 1 week. #microscopy
29/n
![](https://pbs.twimg.com/media/EXal4zrU8AAlXls.jpg)
and breast cancer MØs doi.org/10.1126/scienc…, from @RAFranklin_
33/n
![](https://pbs.twimg.com/media/EXamxskXgAAm1xa.jpg)
You get a medal 🏅
Check out my blog on the paper bit.ly/mammaryDMs
or the full paper bit.ly/35o2XSj. Free access here rdcu.be/b3PJY.
34/n
![](https://pbs.twimg.com/media/EXam6HGVAAANV2g.jpg)
Please ask questions. I have so much time and I'm stuck at home 😁
35/the end *DMs waving
What a great way to cap off this journey.
Can't wait to have a hard copy (I might frame it haha ☺️)
#microscopy #macrophages #3Dimaging #mammarygland
![](https://pbs.twimg.com/media/EXfq6QMUcAIDw6_.jpg)
![](https://pbs.twimg.com/media/EXfu2ciUMAM6ju4.jpg)
![](https://pbs.twimg.com/media/EXfvV91U0AEgjbe.jpg)
Tissue-resident ductal macrophages survey the mammary epithelium and facilitate tissue remodelling @NatureCellBio #SciArt #microscopy #openaccess
![](https://pbs.twimg.com/media/EXi_LPVX0AADUK2.jpg)