Thread by @HenriquesLab

#Pumpy paper is out @NatureComms - "Automating Multimodal Microscopy with NanoJ-Fluidics"! We developed an open-source inexpensive #LEGO-based fluidics system that can be coupled to a microscope to achieve high-performance imaging nature.com/articles/s4146…

We created video abstract with @LaineBioImaging and @christlet that quickly explains paper. #Pumpy enables live-to-fixed imaging, fixation-on-event, super-resolution sequential-labelling (unlim. number protein species), it adds a new dimension to what’s possible at the microscope

@LaineBioImaging @christlet @palmada86 @P_Matos_Pereira did an excellent job making #Pumpy happen. An excellent collaboration with the Charras, @BaumBuzz and @christlet labs. Kudos to @christlet, despite the distance we interacted almost on a daily basis getting the paper together

https://twitter.com/christlet/status/926028281538863104?s=20

@LaineBioImaging @christlet @palmada86 @P_Matos_Pereira @BaumBuzz Also, this was only possible thanks to the support of @MRC_LMCB @UCL @TheCrick
@univamu and our funders - @CNRS @INSB_CNRS @BBSRC @The_MRC @wellcometrust @CRUKresearch

@LaineBioImaging @christlet @palmada86 @P_Matos_Pereira @BaumBuzz @MRC_LMCB @ucl @TheCrick @univamu @CNRS @INSB_CNRS @BBSRC @The_MRC @wellcometrust @CRUKresearch #Pumpy is super-easy to build. We created a @github page explaining all the steps needed to put it together. We traced 23 labs that have already build a copy of the system
github.com/HenriquesLab/N…

@LaineBioImaging @christlet @palmada86 @P_Matos_Pereira @BaumBuzz @MRC_LMCB @ucl @TheCrick @univamu @CNRS @INSB_CNRS @BBSRC @The_MRC @wellcometrust @CRUKresearch @github So... what can #Pumpy do? First thing is live-to-fixed imaging, allowing you to observe a live-sample for few minutes, fix it, then immunostain to observe extra proteins - all automatically by the #LEGO-pumps at the microscope nature.com/articles/s4146… @christlet @LaineBioImaging

Second thing #Pumpy can do - unsupervised experiments, thanks to its programmatic interface. Here a pattern recognition algorithms searches for cells in mitosis then triggering staining. Cells are precisely fixed on same state nature.com/articles/s4146… @christlet @LaineBioImaging

Next we can do live-to-fixed #SuperResolution Microscopy. In this example #Pumpy helps the microscope initiate experiments with #SRRF and then switch to #STORM for increased resolution nature.com/articles/s4146…

@NatureComms @palmada86 @P_Matos_Pereira @SuperResoluSian @BaumBuzz @LaineBioImaging @christlet Also, a note on gender ratio on the paper, it's at 36% (F/F+M). There's scope for improvement and we hope to do so in the future.

@Red_Green_Cow another place where we used red green

@Red_Green_Cow, thank you for your work raising awareness for colour blindness in science data. I'm going to highlight a couple of places where unfortunately we forgot to avoid red green colours. One is this movie