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We're very proud to finally share our work tackling the best way to decode GWAS hits.

biorxiv.org/cgi/content/sh…
1) We use the fundemental regulatory building blocks, promoters, ehancers, and boundaries, to identify key cell types.
2) We then use a combination of pan-genomic and machine learning analyses (developed by the very talented @RonSchwessinger) to predict damaging variants.
3) We use high-resolution NG Capture C (invented by @jojdavies and @jrmmhughes) and statistical modelling to link SNPs to the genes they control.
4) Finally we use CRISPR/Cas9 to directly model the effects of SNP allele in vivo; finding both big and small changes in protein binding, chromatin accessibility, epigenetic modification, and gene expression.
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Eric Trautmann
@EricMTrautmann
Our new preprint is now up! Online decoding of arm reach direction using 2P calcium imaging in NHP with @djoshea, Xulu Sun, @shenoystanford @KarlDeisseroth, @JimMarshel. @samvesuna, @weallen1, Maneesh Sahani, Eyal Seidemann, and many others. biorxiv.org/cgi/content/sh… 1/10
High level goal: Develop 2P calcium imaging for macaque motor cortex. This approach complements electrical recording by enabling 1) dense sampling, 2) genetic targeting, 3) sampling different neurons by moving the lens, 4) access to many neurons simultaneously, and others. 2/10
In addition to showing that we could image neurons during a motor behavior, we also found that we could record from neurons in deep layers by imaging signals from dendrites that project to upper layers. 3/10
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Arif Hamid
@arifahamid
Very excited to share our latest on the organization of activity in dopamine axons in dorsal striatum. We think they are important for reward learning by implementing spatiotemporal credit credit assignment.

biorxiv.org/cgi/content/sh…
What we studied:
1) Scalar dopamine signals are not very useful.
2) There are lots of clues that vector-weighting RPEs can facilitate learning in complex environments.
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What we did:
-> we used an imaging preparation that allows imaging DA axons chronically across multiple anatomical scales.

Notably, we could image the global activity of dopamine across 60-80% of dorsal striatum!
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deWitLab
@deWitLab
Very proud to present the lab’s first bioRxiv preprint: biorxiv.org/cgi/content/sh… Great collaboration between @benoitbruneau and @ElphegeNoraLab. Main gist is that WAPL is important for maintaining dynamic cohesin to bring promoters and enhancers together. Digest in the thread.
@benoitbruneau @ElphegeNoraLab 1/9 Using the auxin inducible degron (AID) system we acute depleted the cohesin release factor WAPL from mouse ESCs. Within 1 hour WAPL is lost. This leads to morphological changes that suggest differentiation. Alkaline phosphatase staining suggests a loss of pluripotency.
@benoitbruneau @ElphegeNoraLab 2/9 RNAseq confirms upregulation of developmental pathways and downregulation of pluripotency related genes. Interestingly, ChIPseq suggests very limited changes in H3K4me3, H3K27ac and H3K27me3.
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Simon Roux
@simroux_virus
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Full disclosure: this project kind of branched out into many directions. I'll try to summarize it in a few tweets, but please feel free to ask for more info on specific points and/or read the preprint to get the full picture ! 1/n
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