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Hannah Reich

@hannahgreich

, 16 tweets, 12 min read

My Authors

Here it is! The TL,DR 🧵 for “Endosymbiotic dinoflagellates pump iron: differences in iron and other trace metal needs among the Symbiodiniaceae”

🔗 (Please reach out if you want a PDF):
link.springer.com/article/10.100…

👏TRACE 👏METALS 👏ARE 👏IMPORTANT👏! Don’t count them out because they’re in far less abundance than C:N:P! They are required as co-factors for virtually all biochemical processes #MetalsInBiology #PhycologyWithoutApology (2/N)

We explore how [Fe] alters growth & trace metal needs of 5 #Symbiodiniaceae species (spanning 3 genera). We highlight how trace metals are interwoven with the physiological inner workings of coral-dinoflagellate symbiosis. #MetalsInBiology #PhycologyWithoutApology (3/N)

We used a diverse set of species (Symbiodinium microadriaticum, S. necroappetens, Breviolum minutum, B. psygmophilum, Effrenium voratum). Represents symbiotic, “necrotrophic,” free-living, tropical, temperate lineages. (4/N)

All culture work was done in a class 100 trace metal clean lab. Lots of acid-washing bottles! #MetalsInBiology #PhycologyWithoutApology (5/N)

First & foremost, #SYMBIODINIACEAE CANNOT GROW WITHOUT IRON! This has implications for Sym distribution & survival to warming. No iron, no growth. Water is also still wet. #MetalsInBiology #PhycologyWithoutApology (6/N)

#Symbiodiniaceae need a lot of iron for growth (relative to other microalgae; i.e. diatoms, Coccolithophores, etc). Their intracellular Fe quotas are also quite large! Lots of co-factors to fulfill that are important for symbiosis, metabolism, #mixotrophy, etc ⚔️🥤🌞 (7/N)

Each species had their own way of coping with exposure to low iron concentrations. Lots of interspecific variation in changes of trace metal (Fe, Zn, Mn, Cu, Mo, etc) content/uptake/efficiency in response to low [Fe]. #MetalsInBiology #PhycologyWithoutApology (8/N)

The variation could be attributed to many things: compensation for low Fe w/ other metals, diff N/P needs, siderophores, non-specific metal uptake, etc. Could have cascading effects metalloenzyme activity (includes N & P transporters)! No shortage of follow up Qs! (9/N)

The metal profiles of #Symbiodiniaceae clustered by ecological guild (e.g., temperate species vs tropical vs ‘necrotrophic’). Some clustering by [Fe] within this! #PhycologyWithoutApology #ThankYouTraceMetals #SoMetal (10/N)

The diff trace metal needs among the #Symbiodiniaceae may be axis of niche diversification. Also may (in part) modulate the regional/local distribution of each spp. Diff metal needs b/t (Sym) spp prob important to meeting metabolic demands of each coral host (11/N) #SoMetal

Final plug before the housekeeping, acknowledgements, etc... Trace metals are so important to coral/sym physiology!!! Really really worth including in our discussions about bleaching resistance #ThankYouTraceMetals #PhycologyWithoutApology #SoMetal (12/N)

Data & code can be found on GitHub! FYI, there is an explanation of each file in the readme. Explains abbreviations used too! (13/N) #ThankYouTraceMetals #PhycologyWithoutApology #SoMetal
github.com/hgreich/Symbio…

@NSF

@NSF

This wouldn’t have been possible without the @NSF #EAPSI program! I learned a ton from working with collaborators in 🇹🇼! To anyone still reading, exchange programs are AWESOME! Sadly #EAPSI is no more but the @ASLO_ORG #LOREX program is a great! aslo.org/lorex/ (14/N)

If you made it this far, THANK YOU!

To see these results discussed in the context of forthcoming follow-up papers, here is the video of my recent PhD defense (DM for slide access): psu.zoom.us/rec/share/vtct…

(15/N, END)!