Here it is! The TL,DR 🧵 for “Endosymbiotic dinoflagellates pump iron: differences in iron and other trace metal needs among the Symbiodiniaceae”
🔗 (Please reach out if you want a PDF):
link.springer.com/article/10.100…
🔗 (Please reach out if you want a PDF):
link.springer.com/article/10.100…
👏TRACE 👏METALS 👏ARE 👏IMPORTANT👏! Don’t count them out because they’re in far less abundance than C:N:P! They are required as co-factors for virtually all biochemical processes #MetalsInBiology #PhycologyWithoutApology (2/N) 
We explore how [Fe] alters growth & trace metal needs of 5 #Symbiodiniaceae species (spanning 3 genera). We highlight how trace metals are interwoven with the physiological inner workings of coral-dinoflagellate symbiosis. #MetalsInBiology #PhycologyWithoutApology (3/N)
We used a diverse set of species (Symbiodinium microadriaticum, S. necroappetens, Breviolum minutum, B. psygmophilum, Effrenium voratum). Represents symbiotic, “necrotrophic,” free-living, tropical, temperate lineages. (4/N)
All culture work was done in a class 100 trace metal clean lab. Lots of acid-washing bottles! #MetalsInBiology #PhycologyWithoutApology (5/N)
First & foremost, #SYMBIODINIACEAE CANNOT GROW WITHOUT IRON! This has implications for Sym distribution & survival to warming. No iron, no growth. Water is also still wet. #MetalsInBiology #PhycologyWithoutApology (6/N)
#Symbiodiniaceae need a lot of iron for growth (relative to other microalgae; i.e. diatoms, Coccolithophores, etc). Their intracellular Fe quotas are also quite large! Lots of co-factors to fulfill that are important for symbiosis, metabolism, #mixotrophy, etc ⚔️🥤🌞 (7/N)
Each species had their own way of coping with exposure to low iron concentrations. Lots of interspecific variation in changes of trace metal (Fe, Zn, Mn, Cu, Mo, etc) content/uptake/efficiency in response to low [Fe]. #MetalsInBiology #PhycologyWithoutApology (8/N)
The variation could be attributed to many things: compensation for low Fe w/ other metals, diff N/P needs, siderophores, non-specific metal uptake, etc. Could have cascading effects metalloenzyme activity (includes N & P transporters)! No shortage of follow up Qs! (9/N)
The metal profiles of #Symbiodiniaceae clustered by ecological guild (e.g., temperate species vs tropical vs ‘necrotrophic’). Some clustering by [Fe] within this! #PhycologyWithoutApology #ThankYouTraceMetals #SoMetal (10/N)
The diff trace metal needs among the #Symbiodiniaceae may be axis of niche diversification. Also may (in part) modulate the regional/local distribution of each spp. Diff metal needs b/t (Sym) spp prob important to meeting metabolic demands of each coral host (11/N) #SoMetal
Final plug before the housekeeping, acknowledgements, etc... Trace metals are so important to coral/sym physiology!!! Really really worth including in our discussions about bleaching resistance #ThankYouTraceMetals #PhycologyWithoutApology #SoMetal (12/N)
Data & code can be found on GitHub! FYI, there is an explanation of each file in the readme. Explains abbreviations used too! (13/N) #ThankYouTraceMetals #PhycologyWithoutApology #SoMetal
github.com/hgreich/Symbio…
github.com/hgreich/Symbio…
If you made it this far, THANK YOU!
To see these results discussed in the context of forthcoming follow-up papers, here is the video of my recent PhD defense (DM for slide access): psu.zoom.us/rec/share/vtct…
(15/N, END)!
To see these results discussed in the context of forthcoming follow-up papers, here is the video of my recent PhD defense (DM for slide access): psu.zoom.us/rec/share/vtct…
(15/N, END)!
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