My Authors
Read all threads
8 years since the first manuscript draft but it's finally here!

The interaction of #Ecoli O157 :H7 and #Salmonella Typhimurium #flagella with host cell #membranes and cytoskeletal components | #Microbiology #Pathogenesis #Thread…
@strain9000 @ArielBlocker @MicrobioSoc This all started many 🌑🌒🌓🌔🌕🌖🌗🌘🌑 ago...

Jo Elvidge and I were looking at the role of #flagella in early infection of #Salmonella and #Ecoli in relevant animal tissue models (🐖🐄)

We spotted what looked like very intimate adherence... E.coli O157:H7 on Bovine terminal rectal cellsSalmonella Typhimurium on porcine epithelial cells
It was a toughie to quantify back then with the tools we had, but every time we looked, we saw it 🤓
We then spent ages trying to see just how close this interaction was - outside or inside?

But with confocal we couldn't look closely enough...
Finally I got a chance to do some super cool correlative light and electron microscopy (#CLEM) at @BristolBiochem

And guess what?!
#Membranes were being deformed by #flagella to form channels - so the flagella looked like they were inside by #confocal, but weren't really... Salmonella flagella bundles technically still outside of the
@BristolBiochem Or where they?! 😮

[Spoiler - we still don't know for sure because it's such a technically tricky thing to observe, but maybe flagella can disrupt host cell membranes?]

#flagella #adherence #Pathogenesis
This thread got a bit bundled up, sorry - here's a link to the rest...
@BristolBiochem And another link to the rest of it - sorry chaps!
Missing some Tweet in this thread? You can try to force a refresh.

Keep Current with Dr Eliza Wolfson

Profile picture

Stay in touch and get notified when new unrolls are available from this author!

Read all threads

This Thread may be Removed Anytime!

Twitter may remove this content at anytime, convert it as a PDF, save and print for later use!

Try unrolling a thread yourself!

how to unroll video

1) Follow Thread Reader App on Twitter so you can easily mention us!

2) Go to a Twitter thread (series of Tweets by the same owner) and mention us with a keyword "unroll" @threadreaderapp unroll

You can practice here first or read more on our help page!

Follow Us on Twitter!

Did Thread Reader help you today?

Support us! We are indie developers!

This site is made by just two indie developers on a laptop doing marketing, support and development! Read more about the story.

Become a Premium Member ($3.00/month or $30.00/year) and get exclusive features!

Become Premium

Too expensive? Make a small donation by buying us coffee ($5) or help with server cost ($10)

Donate via Paypal Become our Patreon

Thank you for your support!