8 years since the first manuscript draft but it's finally here!
The interaction of #Ecoli O157 :H7 and #Salmonella Typhimurium #flagella with host cell #membranes and cytoskeletal components | #Microbiology #Pathogenesis #Thread
microbiologyresearch.org/content/journa…
The interaction of #Ecoli O157 :H7 and #Salmonella Typhimurium #flagella with host cell #membranes and cytoskeletal components | #Microbiology #Pathogenesis #Thread
microbiologyresearch.org/content/journa…
@strain9000 @ArielBlocker @MicrobioSoc This all started many 🌑🌒🌓🌔🌕🌖🌗🌘🌑 ago...
Jo Elvidge and I were looking at the role of #flagella in early infection of #Salmonella and #Ecoli in relevant animal tissue models (🐖🐄)
We spotted what looked like very intimate adherence...
Jo Elvidge and I were looking at the role of #flagella in early infection of #Salmonella and #Ecoli in relevant animal tissue models (🐖🐄)
We spotted what looked like very intimate adherence...
It was a toughie to quantify back then with the tools we had, but every time we looked, we saw it 🤓
We then spent ages trying to see just how close this interaction was - outside or inside?
But with confocal we couldn't look closely enough...
But with confocal we couldn't look closely enough...
Finally I got a chance to do some super cool correlative light and electron microscopy (#CLEM) at @BristolBiochem
And guess what?!
#Membranes were being deformed by #flagella to form channels - so the flagella looked like they were inside by #confocal, but weren't really...
And guess what?!
#Membranes were being deformed by #flagella to form channels - so the flagella looked like they were inside by #confocal, but weren't really...
@BristolBiochem Or where they?! 😮
[Spoiler - we still don't know for sure because it's such a technically tricky thing to observe, but maybe flagella can disrupt host cell membranes?]
#flagella #adherence #Pathogenesis
[Spoiler - we still don't know for sure because it's such a technically tricky thing to observe, but maybe flagella can disrupt host cell membranes?]
#flagella #adherence #Pathogenesis
This thread got a bit bundled up, sorry - here's a link to the rest...
@BristolBiochem And another link to the rest of it - sorry chaps!
@threadreaderapp unroll
