We have just released a new version of the Nextclade web application clades.nextstrain.org for #SARSCOV2. We now use Nextalign (C++ via wasm) under the hood, which allows for many new exacting features!

A quick tour!

1/7
Analysis now takes a few seconds to start, but overall it has become much faster and analyzing hundreds of sequences should be no problem. You can download the reference alignments, including translations of SARS-CoV-2 genes, for further analyses.

2/7
You can now examine the diversity of the nucleotide alignment and alignments of different proteins. Select a gene from the drop-down or click on a gene in the genome annotation panel below.

3/7
The tooltips to explore diversity have become much more informative. For amino acid changes, we now provide a nucleotide context view that is particularly helpful for complex mutations. Consecutive changes are merged into one tooltip.

4/7
A little icon next to the sequence tells you whether some genes of the sequence have failed to translate, either because they weren't covered or because of frame shifts.

5/7
As before, the samples are placed on a reference tree that now includes the updated #Nextstrain clades and a diverse set of lineages.

6/7
Brought to you by the @nextstrain team.

Share your comments and suggestions at discussion.nextstrain.org or github.com/nextstrain/nex… or reach out to @ivan_aksamentov.

7/7

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More from @nextstrain

10 Jun
Thanks to #opendata sharing via @GISAID, we've updated nextstrain.org/ncov with 10836 new #COVID19 #SARSCoV2 sequences!

1/4
You can see new sequences from Australia, Guadeloupe and Mexico on nextstrain.org/ncov/oceania and nextstrain.org/ncov/north-ame….

(Thanks to @SAHealth, @JMPawlotsky, @APHP, @Inserm, @institutpasteur, @todoscicese)

2/4
Check out new sequences from Belarus, Singapore, Taiwan and Vietnam on nextstrain.org/ncov/europe and nextstrain.org/ncov/asia.

(Thanks to RRPCEM Belarus, @mysgh, @Taiwan_CDC, @OUCRU_Vietnam)

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Read 4 tweets
6 Apr
Thanks to #opendata sharing by @GISAID, we've updated nextstrain.org/ncov with 24322 new #COVID19 #SARSCoV2 sequences!

Check out new seqs from Togo (1) & Uganda (120) on nextstrain.org/ncov/africa.

(Thanks to @transvihmi, @ird_fr, @Inserm, @UVRIug, @MRC_Uganda, @LSHTM)

1/14
New sequences from Aruba (36), Bonaire (26), Brazil (368) and Curacao (30) can be found on nextstrain.org/ncov/south-ame….

(Thanks to @rivm, Instituto Adolfo Lutz, @hosp_einstein , @UFCSPA)

2/14
You can see the new sequences from Australia (24) and New Zealand (20) on nextstrain.org/ncov/oceania.

(Thanks to @NSWHPathology @WestSydHealth @Sydney_Uni PHV-FSS #PathWest Laboratory Medicine @WAHealth #CIDMPH @ESRNewZealand @MathStorey @Joepdl @sciolato)

3/14
Read 14 tweets
31 Mar
Thanks to #opendata sharing by @GISAID, we've updated nextstrain.org/ncov with 9739 new #COVID19 #SARSCoV2 sequences!

Check out the new sequences from Australia (14) on nextstrain.org/ncov/oceania.

(Thanks to #RoyalPrinceAlfredHospital, PHV-FSS)

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New sequences from Libya (76), Morocco (2) & Nigeria (1) can be seen on nextstrain.org/ncov/africa.

(Thanks to Genologica Málaga, Laboratoire de Recherche et d'Analyses Medicales de la Gendarmerie Royale, @NCDCgov)

2/14
Seqs from Argentina (769) & Brazil (3) on nextstrain.org/ncov/south-ame….

(Thanks to HRU @CadicUshuaia @UNTDF @intaCIAP Biocódices @gcba @CONICETDialoga @intaargentina LMBA @AcuarioRParana @ProyectoPAIS4, Laboratorio Central Mg. Luis Alfredo Pianciola @MinSaludNeuquen, @fiocruz)

3/14
Read 14 tweets
30 Mar
Thanks to #opendata sharing by @GISAID, we've updated nextstrain.org/ncov with 11073 new #COVID19 #SARSCoV2 seqs!

New seqs from Australia (16) on nextstrain.org/ncov/oceania.

(Thanks to @NSWHPathology, @WestSydHealth, @Sydney_Uni, #PathWest Lab Med, @WAHealth, #CIDMPH)

1/9
Check out the new sequences from India (99), Indonesia (10) and Pakistan (12) on nextstrain.org/ncov/asia.

(Thanks to @icmr_niv, @DBTIndia, #NIMHANS, @DBT_NCCS_Pune, @ILS_Bhubaneswar, NIHRD Indonesia, @NIH_Pakistan)

2/9
You can find the new sequences from Belgium (834) and Czech Republic (100) on nextstrain.org/ncov/europe.

(Thanks to @az_delta @JessaZiekenhuis @umons @GroupeJolimont Rega Institute at @KU_Leuven @FNBrno Institute of Applied Biotechnologies)

3/9
Read 9 tweets
30 Mar
Thanks to #opendata sharing by @GISAID, we've updated nextstrain.org/ncov with 48183 new #COVID19 #SARSCoV2 sequences!

New sequences from Côte d'Ivoire (33) and Kenya (5) can be seen on nextstrain.org/ncov/africa.

(Thanks to CHU Bouaké @KEMRI_Kenya @tagcc_info)

1/20
Check out the new seqs from Angola (71), Nigeria (11) and South Africa (276) on nextstrain.org/ncov/africa. Botswana (1) currently not visible due to random subsampling.

(Thanks to @BotsHarvard @NCDCgov @krisp_news @rjlessells @Tuliodna @EduanWilkinson @nicd_sa @BotsHarvard)

2/20
Check out the new sequences from Australia (34) and French Polynesia (10) on nextstrain.org/ncov/oceania.

(Thanks to #RoyalPrinceAlfredHospital, PHV-FSS, #PathWest Laboratory Medicine, @WAHealth, @JossetLab @Antoninbal)

3/20
Read 20 tweets
24 Mar
Thanks to #opendata sharing by @GISAID, we've updated nextstrain.org/ncov with 10924 new #COVID19 #SARSCoV2 sequences!

Check out the new sequences from Indonesia (1) and Singapore (7) on nextstrain.org/ncov/asia.

(Thanks to @upnjakarta, NCID NPHL Singapore)

1/10
New sequences from Botswana (2), Libya (1) and South Africa (4, currently not visible due to random subsampling) can be found on nextstrain.org/ncov/africa.

(Thanks to @BotsHarvard, Genologica Málaga, @nhls_sa, @nicd_sa)

2/10
New sequences from Australia (27) and Papua New Guinea (43) can be seen on nextstrain.org/ncov/oceania.

(Thanks to PHV-FSS, National Public Health Lab & Centre for Infectious Diseases Singapore, Central Public Health Lab Papua New Guinea)

3/10
Read 10 tweets

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