Thread by @exceedhergrasp1

Hanson thread.
Starts with: "well, I have no conflicts of interest to declare but I am sure I do think differently from some of those @NIH " #fatigue (1/8)
Preach, @DrMaureenHanson

Mentions low blood volume in ME/CFS.
"The fatigue in ME/CFS is not the result of lack of motivation". Many are willing to tolerate pain and fatigue in order to do something important. In fact, we have repeatedly needed to counsel patients not to overdo. #fatigue #MEcfs (2/8)

Abnormal NKC function is one of our most repeatable findings. (3/8) #pwME #MECFS

Hanson's lab has looked at:
~ flow cytometry
~ confocal microscopy
~ Seahorse (a device that analyzes cellular metabolism)
In CD4+, CD8+, and NKCs. (The first two are kinds of T cells.) (4/8) #fatigue #pwME

Some cells had
~Reduced mitochondrial membrane potential
~Reduced utilization of glycolysis at rest
(5/8) #MECFS #pwME

There were differences in fatty acid oxidation, too: (6/8) #pwME #MECFS

"One of the things very frustrating in ME/CFS" is that papers about cytokines often disagree. So we examined how exercise affected the cytokines in plasma & extracellular vesicles. (7/8) #MECFS

Blood was collected before, after, and at 24 hours (PEM)... Cytokine networks significantly differ between HCs and pwME as well as between the different timepoints. RNAseq is a future direction.
Summary: #pwME #fatigue #MECFS
(8/8)

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