Thread by @wilmeslab

Check out our new paper by @smartinezarbas et al. about #CRISPR mediated interactions within a wastewater #microbiome in @NatureMicrobiol! Exciting collaboration between @uni_lu @TGen @IUBloomington @isbsci @KU_Leuven. Funding by @FnrLux
nature.com/articles/s4156…

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In parallel with recent work of @mhe345 @NatureComms nature.com/articles/s4146…, we explored the roles of #plasmids and #phages within a wastewater treatment plant microbial community, using longitudinal integrated #omics data 2/9

We identified 31 bacterial MAGs with complete #CRISPR-Cas systems from the 14-months data set. These MAGs served as the basis for downstream population-level analyses of #CRISPR interactions 3/9

We found #plasmid sequences outnumber #phage sequences in both absolute number and in terms of #CRISPR targeting! Models show plasmids as strong drivers of dominant #population dynamics 4/9

Linking #phage sequences to their hosts, tracking the resulting network over time, and … 5/9

comparing to the #plasmid-host network revealed that it is denser than the #phage network! Moreover, #plasmids containing #AMR genes were less targeted compared to those without. 6/9

The dominant population Microthrix parvicella exhibited an active #CRISPR system because it had spacer integration/deletion events and 6 #Cas #genes and #proteins expressed over time 7/9

Thanks to all those involved, among others, @shaman_ns @mhe456 @EmilieMuller01 @claczny @BenoitKunath @LanceBPrice @Karoline_Faust @r_l_moritz @PaulKeim 8/9

Raw data and code available!!

MT+MG: ncbi.nlm.nih.gov/bioproject/230…

MP: ebi.ac.uk/pride/archive/…

github.com/susmarb/LAO_mu…

git-r3lab.uni.lu/shaman.narayan…

#openscience #openaccess

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