Discover and read the best of Twitter Threads about #phage
Most recents (12)
Unprecedented case series advances promise of #phage therapy
👇
Phage Therapy of #Mycobacterium Infections: Compassionate-use of Phages in 20 Patients with Drug-Resistant Mycobacterial Disease
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academic.oup.com/cid/advance-ar…
@MariadelMarTom
👇
Phage Therapy of #Mycobacterium Infections: Compassionate-use of Phages in 20 Patients with Drug-Resistant Mycobacterial Disease
1/
academic.oup.com/cid/advance-ar…
@MariadelMarTom
Treatment of Non-tuberculous Mycobacterium could be challenging due to intrinsic antibiotic #resistance. Bacteriophage therapy represents a potentially novel approach.
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Phages were administered intravenously, by aerosolization, or both to 20 patients
Favorable clinical or microbiological responses were observed in 11 patients.
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Favorable clinical or microbiological responses were observed in 11 patients.
3/
Very pleased to share our ✨New Paper✨ about an epigenetic conflict between a restriction–modification system and a methylation-dependent nuclease.
Want to know how that pans out? Continue reading here 🧵👇🏻 or head over to @NAR_Open for the full story: academic.oup.com/nar/advance-ar…
Want to know how that pans out? Continue reading here 🧵👇🏻 or head over to @NAR_Open for the full story: academic.oup.com/nar/advance-ar…
It all began when we started investigating the anti-#CRISPR repressor Aca2 from Pectobacterium #phage ZF40. Back then, we stumbled upon an RM system in Pecto that inhibited transformation and conjugation and completely blocked phage infection. 🧬🚫 [2/n]
Because this strain could only take up DNA with RM-compatible methylation, we knocked out the restriction endonuclease of the RM system, enabling us to pursue our anti-CRISPR regulation project:
academic.oup.com/nar/article/47…
And that could have been the end of it... [3/n]
academic.oup.com/nar/article/47…
And that could have been the end of it... [3/n]
We are delighted to announce the release of #PhageAI 0.10.0: extended repository and the new #phage #prediction tool: #chronic #lifecycle. 1/4
Each of the #phage genome has computed a new numerical vector representation by #Phage2Vec technology, re-classified #lifecycles and re-computed nearest neighborhood what introduced updated alignment-free top-10 similar #bacteriophages registry. 2/4
Furthermore, we released beta #bacteriophage #taxonomy #prediction tool.
Feel free to extend your account role from basic to Expert user using "My Profile" view, to access it from the @PhageAI. 3/4
Feel free to extend your account role from basic to Expert user using "My Profile" view, to access it from the @PhageAI. 3/4
#Phage are often only seen as bacterial predators, but they’re also major drivers of bacterial evolution through generalised transduction.
In this new preprint, we combine lab work & mathematical modelling to reveal the dynamics of this dual nature.
1/n
biorxiv.org/content/10.110…
In this new preprint, we combine lab work & mathematical modelling to reveal the dynamics of this dual nature.
1/n
biorxiv.org/content/10.110…
Generalised transduction happens when a replicating phage mistakenly packages non-phage DNA. The resulting transducing phage can inject this DNA in another bacteria, as shown in the Figure.
This is notably a powerful way to transfer plasmids, major vectors of #AMR genes.
2/n
This is notably a powerful way to transfer plasmids, major vectors of #AMR genes.
2/n
We co-cultured two single resistant strains of S. aureus (B_E and B_T) with 80alpha phage (P_L).
Here, horizontal gene transfer can only happen by generalised transduction, so if we detect double resistant bacteria (B_ET), we know that transduction happened.
3/n
Here, horizontal gene transfer can only happen by generalised transduction, so if we detect double resistant bacteria (B_ET), we know that transduction happened.
3/n
The "missing" scientist from #WIV seems to be someone who was in the #phage lab 5 years ago?
That's not a common enough name that there's been two at the same institute?
A phage is about as unlike a #coronavirus as you can get while still being a virus.
researchgate.net/scientific-con…
That's not a common enough name that there's been two at the same institute?
A phage is about as unlike a #coronavirus as you can get while still being a virus.
researchgate.net/scientific-con…
The lab started some stuff on African Swine Fever, but not till after she left.
And a couple of papers on SARS-COV-2 that seem to be adapting methods they developed with other pathogens, after the pandemic was discovered.
And a couple of papers on SARS-COV-2 that seem to be adapting methods they developed with other pathogens, after the pandemic was discovered.
Even the tabloids reporting it found she left town 4 years before the pandemic started.
Check out our new paper by @smartinezarbas et al. about #CRISPR mediated interactions within a wastewater #microbiome in @NatureMicrobiol! Exciting collaboration between @uni_lu @TGen @IUBloomington @isbsci @KU_Leuven. Funding by @FnrLux
nature.com/articles/s4156…
A 🧵👇
1/9
nature.com/articles/s4156…
A 🧵👇
1/9
In parallel with recent work of @mhe345 @NatureComms nature.com/articles/s4146…, we explored the roles of #plasmids and #phages within a wastewater treatment plant microbial community, using longitudinal integrated #omics data 2/9
Here it is!
#Inktober2020 day 1 in my #PathogenPortraits series. After years of studying this in cow bums, it had to be #EcoliO157
It causes bloody diarrhoea & if that wasn't bad enough, sometimes kidney failure & death. More info @
nhsinform.scot/illnesses-and-…
#sciart #microbiology
#Inktober2020 day 1 in my #PathogenPortraits series. After years of studying this in cow bums, it had to be #EcoliO157
It causes bloody diarrhoea & if that wasn't bad enough, sometimes kidney failure & death. More info @
nhsinform.scot/illnesses-and-…
#sciart #microbiology
#Inktober2020 day 2 in my #PathogenPortraits series. 'The Clap' (Neisseria gonorrhoeae) b/c who doesn't 💚 green purulent discharge?
It's sexually transmitted but also from mother->newborn during birth to cause blindness.
More info: microbewiki.kenyon.edu/index.php/Neis…
#sciart #microbiology
It's sexually transmitted but also from mother->newborn during birth to cause blindness.
More info: microbewiki.kenyon.edu/index.php/Neis…
#sciart #microbiology
#Inktober2020 day 3 in my #PathogenPortraits series. The very 💛golden💛 #MRSA.
Minds it's own business on your skin, but if it gets inside, methicillin won't do a thing to stop it.
More info at mrsaactionuk.net/MRSA.html #sciart #microbiology
Minds it's own business on your skin, but if it gets inside, methicillin won't do a thing to stop it.
More info at mrsaactionuk.net/MRSA.html #sciart #microbiology
Study in mice finds that commensal #viruses (eukaryotic + bacteriophage) are essential for the homeostasis of intestinal intraepithelial lymphocytes (IELs) in a manner that could impact IBD development: nature.com/articles/s4159…
2/2 The results support this recent study which found that during (mouse) colitis the intestinal #phage population is altered and transitions from an ordered state to a stochastic dysbiosis: ncbi.nlm.nih.gov/m/pubmed/30038…
3/3 The study is also yet another example of how phage interact w/ the mammalian #immune response. More background on that topic here: ncbi.nlm.nih.gov/pmc/articles/P…
Microbes/viruses interact to drive disease 👉 Here, a #bacteriophage (Pf) that infects bacterial pathogen p. aeruginosa (Pa)☝️promoted Pa virulence (in wounds) by triggering maladaptive anti-viral responses in human cells + suppressing bacterial clearance: science.sciencemag.org/content/363/64…
In addition 👉 1. The data suggest that a natural (unmodified) #bacteriophage may be able to produce mRNA within human cells☝️2. Consistent with a pathogenic role for Pf #phage, a vaccine against Pf phage protected against P. aeruginosa wound infections in mice
Simple summary 👉 This marks the first time a #bacteria-infecting #virus, otherwise known as a #bacteriophage...has been observed inducing the immune system to mount an antiviral response and, in doing so, causing it to ignore the bacterial infection: google.com/amp/s/medicalx…
When I say #phage, do you think bacterial parasite? Superbug slayer (👀 @chngin_the_wrld)? #PhageTherapy?
Just out in @sciencemagazine, @bollyky and I find phage PROMOTE bacterial infection by directly triggering a maladaptive anti-viral immune response.
science.sciencemag.org/content/363/64…
Just out in @sciencemagazine, @bollyky and I find phage PROMOTE bacterial infection by directly triggering a maladaptive anti-viral immune response.
science.sciencemag.org/content/363/64…
ABSTRACT: Pseudomonas aeruginosa (Pa) is a multidrug-resistant Gram-negative bacterium commonly found in health care settings. Pa infections frequently result in considerable morbidity and mortality.
We find that a type of temperate filamentous bacteriophage that infects and integrates into Pa is associated with chronic human wound infections. Likewise, wounds in mice colonized with phage-infected Pa were more severe and longer-lasting than those colonized by Pa alone.
Team searches for prokaryotic small #proteins in #HMP dataset that are traditionally hard to detect 👉 Describe more than 4000 conserved protein families, most of which are novel (90% have no known domain, and ~1/2 are not represented in reference genomes):biorxiv.org/content/early/…
Small #proteins identified by the team are 👉 potentially involved in crosstalk w/ the host or other #microbial cells, may be subject to horizontal #gene transfer...and may be related to defense against #phage or other bacteria (among other functions!): biorxiv.org/content/biorxi…
Also, important previous discoveries by Ami S Bhatt and @kyrpides (both authors of the small protein paper) 👉 are featured in my recent article: “My favorite 2018 #papers that impact the study of chronic inflammatory disease”☝️Check them out: microbeminded.com/2018/12/30/myf…
Disillusionment? No. 👉 Just last year, @Stanford’s Steven Quake identified over 3000 previouly undiscovered #viruses, #bacteria and fungal organisms in the #blood of immunocompromised subjects☝️Using cell-free #DNA sequencing that accounted for contamination...
Then concluded the paper by stating that the novel #organisms 👉 “...have potential consequences for human #health. They may prove to be the cause of acute or chronic #diseases that, to date, have unknown etiology…”☝️What hope for #microbiome-based therapies!
Also, as #microbiome researcher, I am incredibly encouraged by the explosion of human #phage + #virome research in just the past years (+ associated tools for novel #virus identification) 👉 Plus, better #archea-targeting methodologies like those used here:biorxiv.org/content/early/…
