Discover and read the best of Twitter Threads about #phage

Most recents (12)

Unprecedented case series advances promise of #phage therapy
Phage Therapy of #Mycobacterium Infections: Compassionate-use of Phages in 20 Patients with Drug-Resistant Mycobacterial Disease
Treatment of Non-tuberculous Mycobacterium could be challenging due to intrinsic antibiotic #resistance. Bacteriophage therapy represents a potentially novel approach.
Phages were administered intravenously, by aerosolization, or both to 20 patients
Favorable clinical or microbiological responses were observed in 11 patients.
Read 5 tweets
Very pleased to share our ✨New Paper✨ about an epigenetic conflict between a restriction–modification system and a methylation-dependent nuclease.

Want to know how that pans out? Continue reading here 🧵👇🏻 or head over to @NAR_Open for the full story:…
It all began when we started investigating the anti-#CRISPR repressor Aca2 from Pectobacterium #phage ZF40. Back then, we stumbled upon an RM system in Pecto that inhibited transformation and conjugation and completely blocked phage infection. 🧬🚫 [2/n]
Because this strain could only take up DNA with RM-compatible methylation, we knocked out the restriction endonuclease of the RM system, enabling us to pursue our anti-CRISPR regulation project:…

And that could have been the end of it... [3/n]
Read 12 tweets
We are delighted to announce the release of #PhageAI 0.10.0: extended repository and the new #phage #prediction tool: #chronic #lifecycle. 1/4
Each of the #phage genome has computed a new numerical vector representation by #Phage2Vec technology, re-classified #lifecycles and re-computed nearest neighborhood what introduced updated alignment-free top-10 similar #bacteriophages registry. 2/4
Furthermore, we released beta #bacteriophage #taxonomy #prediction tool.

Feel free to extend your account role from basic to Expert user using "My Profile" view, to access it from the @PhageAI. 3/4
Read 4 tweets
#Phage are often only seen as bacterial predators, but they’re also major drivers of bacterial evolution through generalised transduction.

In this new preprint, we combine lab work & mathematical modelling to reveal the dynamics of this dual nature.

Generalised transduction happens when a replicating phage mistakenly packages non-phage DNA. The resulting transducing phage can inject this DNA in another bacteria, as shown in the Figure.

This is notably a powerful way to transfer plasmids, major vectors of #AMR genes.

We co-cultured two single resistant strains of S. aureus (B_E and B_T) with 80alpha phage (P_L).

Here, horizontal gene transfer can only happen by generalised transduction, so if we detect double resistant bacteria (B_ET), we know that transduction happened.

Read 12 tweets
The "missing" scientist from #WIV seems to be someone who was in the #phage lab 5 years ago?

That's not a common enough name that there's been two at the same institute?

A phage is about as unlike a #coronavirus as you can get while still being a virus.…
The lab started some stuff on African Swine Fever, but not till after she left.

And a couple of papers on SARS-COV-2 that seem to be adapting methods they developed with other pathogens, after the pandemic was discovered.
Even the tabloids reporting it found she left town 4 years before the pandemic started. Image
Read 3 tweets
Check out our new paper by @smartinezarbas et al. about #CRISPR mediated interactions within a wastewater #microbiome in @NatureMicrobiol! Exciting collaboration between @uni_lu @TGen @IUBloomington @isbsci @KU_Leuven. Funding by @FnrLux…

A 🧵👇

In parallel with recent work of @mhe345 @NatureComms…, we explored the roles of #plasmids and #phages within a wastewater treatment plant microbial community, using longitudinal integrated #omics data 2/9
We identified 31 bacterial MAGs with complete #CRISPR-Cas systems from the 14-months data set. These MAGs served as the basis for downstream population-level analyses of #CRISPR interactions 3/9
Read 9 tweets
Here it is!

#Inktober2020 day 1 in my #PathogenPortraits series. After years of studying this in cow bums, it had to be #EcoliO157

It causes bloody diarrhoea & if that wasn't bad enough, sometimes kidney failure & death. More info @…
#sciart #microbiology Inktober2020 E. coli O157 c...
#Inktober2020 day 2 in my #PathogenPortraits series. 'The Clap' (Neisseria gonorrhoeae) b/c who doesn't 💚 green purulent discharge?

It's sexually transmitted but also from mother->newborn during birth to cause blindness.
More info:…

#sciart #microbiology Image
#Inktober2020 day 3 in my #PathogenPortraits series. The very 💛golden💛 #MRSA.

Minds it's own business on your skin, but if it gets inside, methicillin won't do a thing to stop it.

More info at #sciart #microbiology Methicillin resistant staph...
Read 34 tweets
Study in mice finds that commensal #viruses (eukaryotic + bacteriophage) are essential for the homeostasis of intestinal intraepithelial lymphocytes (IELs) in a manner that could impact IBD development:… ImageImage
2/2 The results support this recent study which found that during (mouse) colitis the intestinal #phage population is altered and transitions from an ordered state to a stochastic dysbiosis:… Image
3/3 The study is also yet another example of how phage interact w/ the mammalian #immune response. More background on that topic here:… Image
Read 3 tweets
Microbes/viruses interact to drive disease 👉 Here, a #bacteriophage (Pf) that infects bacterial pathogen p. aeruginosa (Pa)☝️promoted Pa virulence (in wounds) by triggering maladaptive anti-viral responses in human cells + suppressing bacterial clearance:…
In addition 👉 1. The data suggest that a natural (unmodified) #bacteriophage may be able to produce mRNA within human cells☝️2. Consistent with a pathogenic role for Pf #phage, a vaccine against Pf phage protected against P. aeruginosa wound infections in mice
Simple summary 👉 This marks the first time a #bacteria-infecting #virus, otherwise known as a #bacteriophage...has been observed inducing the immune system to mount an antiviral response and, in doing so, causing it to ignore the bacterial infection:…
Read 3 tweets
When I say #phage, do you think bacterial parasite? Superbug slayer (👀 @chngin_the_wrld)? #PhageTherapy?

Just out in @sciencemagazine, @bollyky and I find phage PROMOTE bacterial infection by directly triggering a maladaptive anti-viral immune response.…
ABSTRACT: Pseudomonas aeruginosa (Pa) is a multidrug-resistant Gram-negative bacterium commonly found in health care settings. Pa infections frequently result in considerable morbidity and mortality.
We find that a type of temperate filamentous bacteriophage that infects and integrates into Pa is associated with chronic human wound infections. Likewise, wounds in mice colonized with phage-infected Pa were more severe and longer-lasting than those colonized by Pa alone.
Read 4 tweets
Team searches for prokaryotic small #proteins in #HMP dataset that are traditionally hard to detect 👉 Describe more than 4000 conserved protein families, most of which are novel (90% have no known domain, and ~1/2 are not represented in reference genomes)… ImageImageImageImage
Small #proteins identified by the team are 👉 potentially involved in crosstalk w/ the host or other #microbial cells, may be subject to horizontal #gene transfer...and may be related to defense against #phage or other bacteria (among other functions!):… Image
Also, important previous discoveries by Ami S Bhatt and @kyrpides (both authors of the small protein paper) 👉 are featured in my recent article: “My favorite 2018 #papers that impact the study of chronic inflammatory disease”☝️Check them out:… ImageImage
Read 3 tweets
Disillusionment? No. 👉 Just last year, @Stanford’s Steven Quake identified over 3000 previouly undiscovered #viruses, #bacteria and fungal organisms in the #blood of immunocompromised subjects☝️Using cell-free #DNA sequencing that accounted for contamination...
Then concluded the paper by stating that the novel #organisms 👉 “...have potential consequences for human #health. They may prove to be the cause of acute or chronic #diseases that, to date, have unknown etiology…”☝️What hope for #microbiome-based therapies! ImageImageImageImage
Also, as #microbiome researcher, I am incredibly encouraged by the explosion of human #phage + #virome research in just the past years (+ associated tools for novel #virus identification) 👉 Plus, better #archea-targeting methodologies like those used… ImageImage
Read 3 tweets

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