Thread by @Em23397

Happy to be a co-author of this article about #viralepersistence in #longCovid patients-found by dogs of @Env_Alfort- w/ Dominique GRANDJEAN, Dorsaf SLAMA, Capucine GALLET, Clothilde JULIEN, Marc BLONDOT, Maissa BENAZAZIEZ, Judith ELBAZ and Dominique SALMON #apresJ20 #Covid19

https://twitter.com/medrxivpreprint/status/1481319554554937349

Dogs have already demonstrated high capacity in detecting Covid19 infection in acute phase (very high sensitivity)
Now they help demonstrating once more that #viralepersistence is present « in some Long Covid patient »..#apresJ20 #longCovid #Covidlong #Covid19 👇

I would like to explain why we wrote « at least in some Long Covid patients » : dogs positively discriminated half of the 45 people but there are surely much more #longCovid #Covidlong positive = with #viralepersistence #persistancevirale #apresJ20 👇

Indeed, samples of #apresJ20 #longCovid patients were taken home sent by postmail : the quality of sampling as well as the postmail delays may have affected the préservation of the samples, which we specified here 👇

«In this study with Long COVID patients, it is difficult to interpret negative discriminations as
the sample quality take at home may have varied, and postal delays may have altered the preservation.
Therefore, the sensitivity of canine olfactory test might be underestimated👇

Nevertheless, in this study, the specificity of SARS -CoV-2 olfactory detection by dogs is
strikingly high, like in previous studies »

[…]
👇

« In conclusion, these results confirm the high probability of a SARS-CoV-2 #viralpersistence at least for some #LongCOVID patients, possibly with virus actively replicating 👇#apresJ20 #CovidLong #Covid19

It also shows the limited utility of serological tests made during #LongCOVID. Such conclusions are of major importance for the management of future Long COVID treatments trials » #TreatLongCovid !!!! #Covid19 #apresJ20 #persistancevirale #viralpersistence 🐶🐕

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Our article about #viralpersistence in #longCovid detected by dogs has been published!

longdom.org/open-access/sc…

longdom.org/clinical-trial…

Great collaboration with @dgrandjean at @Env_Alfort , Pr Dominique Salmon @HotelDieuParis of course,…👇
1/n
#Covidlong #Covid19 #apresJ20

..Dorsaf Slama, Capucine Gallet, Clothilde Julien, Marc Blondot, Judith Elbaz, Maissa Benazaiez, Friederike Twele, Holger Andreas Volk
2/n
#LongCovid #Covidlong #Covid19 #apresJ20 #viralpersistence #ResearchLongCovid #TreatLongCovid @ActionCovidLong @apresj20 @Notelet13

« ABSTRACT
Background:Dogs can be trained to identify several substances not detected by humans, corresponding to specific VOCs.The presence of VOCs, triggered by #SARSCoV2 infection,was tested in sweat from #longCovid patients
3/n
#Covidlong #Covid19 #apresJ20 #TreatLongCovid

Results: 45 #longCovid patients, mean age 45, 73.3% female, with prolonged symptoms evolving for a mean of 15.2 months (5-22) were tested. Dogs discriminated in a positive way 23/45 (51.1%) long COVID patients versus 0/188 (0%) control healthy individuals (p<.0001)
4/n
#apresJ20

Conclusion: Our data provide arguments for the persistence of viral antigens at least in some #longCovid patients and raise the possibility of future therapeutic options »
5/n
#Covidlong #Covid19 #apresJ20 #TreatLongCovid #ResearchLongCovid

Extracts of the Discussion :
“These results strongly suggest that #SARS-CoV-2 may persist at least in some #LongCovid patients. Furthermore, they show that dogs can detect VOCs in some patients with a negative #SARS-CoV-2 serology” 6/n
#Covidlong #Covid19 #apresJ20

“In this study with #longcovid patients, it’s difficult to interpret negative discriminations as the sample quality taken at home may have varied, and postal delays may have altered the preservation.Therefore,the sensitivity of canine olfactory test might be underestimated”
7/n

“This canine detection test, with detection of VOCs, supports the hypothesis that the virus is still actively replicating.”
8/n
#longCovid is #Covid19 active infection !!! #Covidlong #Covid19 #apresJ20 #TreatLongCovid #ResearchLongCovid

« This very sensitive and non-invasive technique, which can be used both during acute and extended phases, is also faster than the RT-PCR and provides a holistic information…
9/n
#longCovid is an active I#Covid19 nfection #Covidlong #apresJ20 #TreatLongCovid #ResearchLongCovid

…Indeed, it gives reliable results on the existence of a persistent infection, even when the precise location of the virus reservoir in the body is not known.” 10/n
#longCovid is #COVID19 active infection #Covidlong #apresJ20 #TreatLongCovid #ResearchLongCovid

“CONCLUSION
These results reinforce the hypothesis of a #SARSCoV2 antigens persistence at least for some #longCovid patients, possibly w/ virus actively replicating. It also shows the limited utility of (..) serological tests made during #longCovid. …
11/n
#Covid19 #apresJ20

..Such results raise the possibility of future therapeutic options and invite to build #longCovid #antiviral #treatments trials…
12/n
#Covidlong is #Covid19 active infection #apresJ20 #ResearchLongCovid #TreatLongCovid

…In addition, w/ a better characterization of the detected VOCs, an improvement of odor sampling methods and the development of point-of-care instruments, this detection by dogs could also help to implement scent-based tests for other major human pathogens.” n/n
#longCovid

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