I can't even drag myself out of bed at 4:45am when I'm healthy.
All this waiting around for tests while still at home is the worst possible way to contain a virus, unless every family member is totally isolated.
All this waiting around for tests while still at home is the worst possible way to contain a virus, unless every family member is totally isolated.
If we don't have enough qPCR to handle more than 1000 tests per week nationwide, at this point - we should be requesting, if not mandating, that high throughput sequencing facilities shift to at least 30-40% of capacity run genotyping or sequencing of samples.
Send samples to both qPCR facility and seq lab; If positive result from first lab, report back to patient. If negative result, report inconclusive - sample should already be in queue for rtPCR testing, meaning there's the potential to get results a day or two off queuing time.
And, FFS. It appears there are commercially available antibodies already, so get a move on setting up ELISA and immunomagnetic assays, or ...
prosci-inc.com/sars-cov-2-cov…
prosci-inc.com/sars-cov-2-cov…
OH JFC look at this.
Michigan residents: avoid if you can.
This is Betsy Devos's company.
back on subject:
Michigan residents: avoid if you can.
This is Betsy Devos's company.
back on subject:
flow cytometry. electron microscopy.
To detect a simple positive result, you can go in so many directions.
If the hardware is the bottleneck, try pooling samples - 5:1, 10:1, depending on predicted rate of positive results - if pooled sample has +, then run each individually.
To detect a simple positive result, you can go in so many directions.
If the hardware is the bottleneck, try pooling samples - 5:1, 10:1, depending on predicted rate of positive results - if pooled sample has +, then run each individually.
Start thinking in terms of sample triage, not just patient triage (although pt triage should come first, for sure).
Use pooling for lower-probability and/or asymptomatic exposure-based cases. Or run straight PCR, use different probes, and multiplex on sequencers.
Use pooling for lower-probability and/or asymptomatic exposure-based cases. Or run straight PCR, use different probes, and multiplex on sequencers.
Maybe there is someone managing a multi-faceted approach like this.... maybe the single approach is just the face that's put on it for the public, to appear authoritative.
but I doubt it.
And, to me, it just seems stupid and shortsighted.
but I doubt it.
And, to me, it just seems stupid and shortsighted.
And why every single public health department doesn't have their own nanopore array yet is just beyond me. You can use that shiz for food safety testing, water quality testing, genotyping, as well as straight up medical tests, etc.
AND THEY're ONLY $1000.
AND THEY're ONLY $1000.
OKAY, done ranting. For now. But more to come. Esp. on magnetic microbead-based assays.
