Thread by @angryhacademic

recap of a lil #hydration #sciencefromhome #experiment me & some mates ran over the last ~year. analysis not finished, but will release #bloodsugar data before H4H conf starts🤓

Pt 1. A fair while back we had a rather exciting delivery...What have we been up to?1/

Pt 2. After the rather exciting delivery (and some more) arrived, the study design needed to be finalised:

Non-randomised controlled study where we *planned* for experimenters to do the CONTROL arm first, followed by the INTERVENTION arm

🤔
2/
#hydration #sciencefromhome

Pt 3. Equipment ordered & study designed.

Minor problem: i hadnt cannulated in > 1 y & my mates had never learnt at all

Cue odd evening remembering/teaching cannulation on poor Snuffles belly...

*GULP*
My ❤️of #science > my fear of needles it seems
3/
#sciencefromhome #hydrate

Pt 4.ok #hydration #scientists, sneak-a-peak at some results. 2 self-experimenters (a & b) urine volume over a ~7 h test period: 1st morning void > OGTT > 4 h post-OGTT.Any ideas what we did to do this to urine vol?or why outcomes are so diverse between 2 ppl? 3/
#sciencefromhome

Pt 4b. Another way to look at urine vol. We oft ignore time trends in output, but in this exp they tell an interesting story, not exactly what we predicted either.Any ideas?
*on reflection in some ways it mightve been better to standardise times 5/
#hydration #sciencefromhome

Pt 5. Body mass. Couple this with the time trend urine volume data (Pt 4b, below) to get a very interesting picture... distinct and time-trended body #water retention = ↑ AVP? 🤔
6/
#hydration #sciencefromhome

Pt 6. Thirst ratings (100 mm visual analogue scales). One of us got very thirst, the other's #thirst didn't really change.

shouldnt ↑ AVP = ↑ thirst (via ↑ osmolality)??

Any ideas what our experiment was? 7/

#hydration #health #sciencefromhome

Pt 7. Whole blood #glucose response. Again, one "responder" and one "non-responder" = if AVP did ↑ in both experimenters, then something else must be driving hyperglycaemia...?? but what? 🤔

#hydration #health #sciencefromhome

Pt 8. Just for the giggles, here I am breaking all normal lab rules infusing my own saline 😱

I think i love #science more than is legally recommended 🤪

#hydration #health #sciencefromhome