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Microscopy technique development's exploding, but core facilities still buy confocals. This ugly baby will end that.
Less phototoxicity, WAY faster than confocal. NO DRAWBACK.
Details here: andrewgyork.github.io/high_na_single…
Co-invented by @amsikking.
Get one from jon@asiimaging.com
Less phototoxicity, WAY faster than confocal. NO DRAWBACK.
Details here: andrewgyork.github.io/high_na_single…
Co-invented by @amsikking.
Get one from jon@asiimaging.com
I've heard there's "no free lunch" in microscopy; advantages come with tradeoffs. We take this as a personal challenge!
Our new microscope design gives the gentleness/speed of lightsheet microscopy, AND the high NA and simple sample prep you're used to with confocal microscopy.
Our new microscope design gives the gentleness/speed of lightsheet microscopy, AND the high NA and simple sample prep you're used to with confocal microscopy.
We want this microscope in YOUR hands, ASAP. Traditional publication takes years, commercialization even longer. Even preprints take time to prepare.
Our fastest path is #OpenScience ; see
andrewgyork.github.io/high_na_single…
for up-to-date parts list, build instructions, & preliminary data
Our fastest path is #OpenScience ; see
andrewgyork.github.io/high_na_single…
for up-to-date parts list, build instructions, & preliminary data
Our design uses ALMOST all stock parts. There's one custom lens, invented at Calico, manufactured by @SpecialOptics, and distributed by @ASIImaging.
Help ASI commission a large (cheap!) batch by telling @jsdaniel02 you're interested! Email: jon@asiimaging.com
Help ASI commission a large (cheap!) batch by telling @jsdaniel02 you're interested! Email: jon@asiimaging.com
(...also we really need help naming this objective. I can't keep calling it "Mr. Snouty" forever, right?)
Our rapid dissemination approach is in same spirit of improving scientific publishing which you've heard me talking about before:
Of course, we're standing on the shoulders of giants.
It all started with Botcherby, Wilson, et al.'s seminal paper on remote refocusing (doi.org/10.1016/j.optc…).
A few years ago we made our own, and shared the design:
It all started with Botcherby, Wilson, et al.'s seminal paper on remote refocusing (doi.org/10.1016/j.optc…).
A few years ago we made our own, and shared the design:
And we've always found Dunsby's OPM (doi.org/10.1364/OE.16.…) clever as hell.
(Hey @LD_light_ and @NBioimaging : is Chris on Twitter? Also, @amsikking and I think your lab is awesome.)
(Hey @LD_light_ and @NBioimaging : is Chris on Twitter? Also, @amsikking and I think your lab is awesome.)
So when we saw the @biorxivpreprint from @Bin_YANG_Optics et al. back in 2018 (doi.org/10.1101/273359), it blew our frigging minds.
They *shattered* the NA limit of the Dunsby design!
They *shattered* the NA limit of the Dunsby design!
Special thanks to Bin, Bo, and their team at @UCSF for preprinting. The lag between preprint and "traditional" publication was more than a year! @biorxivpreprint got us building on their work immediately.
Also shoutout to Bouchard, Hillman et al. (doi.org/10.1038/nphoto…) and Kumar, Kozorovitskiy et al. (arxiv.org/abs/1804.09844), for their elegant scanning. At first I thought the SCAPE paper was a misprint. You can't scan Z with a galvo! (Turns out you can. It's super fast.)
Finally, a big shoutout to @amsikking. Alfred's an awesome optical engineer (and physicist, microscopist, builder, colleague, friend...). I'm lucky to work with him at Calico.
He's also new to Twitter. You should follow him!
He's also new to Twitter. You should follow him!
