me64 陳美滿 Profile picture
studied chemical eng, https://t.co/RBrdPaQZTe https://t.co/JlVAmWf8kj… https://t.co/gaM7krG1Nl…
Dec 12, 2021 23 tweets 7 min read
A 🧵about 🧠problems after C19 💉

1. Neurological complications after first dose of COVID-19 vaccines and SARS-CoV-2 infection.
“There was an increased risk of Guillain–Barré syndrome and Bell’s palsy with ChAdOx1nCoV-19, hemorrhagic stroke with BNT162b2. ..An independent Scottish cohort provided further support for the association between ChAdOx1nCoV and Guillain–Barré syndrome, there was a substantially higher risk of all neurological outcomes in the 28 days after a positive SARS-CoV-2 test including Guillain–Barré syndrome. Overall, we
Nov 23, 2021 14 tweets 6 min read
Thyroid as a #target of adjuvant #autoimmunity /#infammatory syndrome due to mRNA‐based SC2 vaccination.
#Vaccines contain #adjuvants which are not exempt from adverse efects and can #trigger the autoimmune/infammatory syndrome induced by adjuvants (#ASIA). Image Multiple endocrine organs have receptors for ACE2.
mRNA of ACE2, the SARS-CoV2 receptor, is expressed in the thyroid cells.
ACE2 receptor is abundantly expressed by the thyroid. Elevated levels of proinflammatory cytokines could increase the expression ofACE2 on the thyroidgland.
Nov 9, 2021 8 tweets 3 min read
Road to #Paxlovid #Pfizer #COVID19 new drug. pubmed.ncbi.nlm.nih.gov/34029993/
3CLpro inhibitors commonly target host proteases, which presents a potential liability for unexpected side effects. Challenges : enzyme inhibition was only achieved in the absence of physiologically relevant reducing agents, and the reported antiviral effect in cells infected with SARS-CoV-2 could not be reproduced and was therefore suspected to be an indirect consequence of cell death.
Nov 9, 2021 22 tweets 6 min read
The Road to Paxlovid (PF-07321332)
A thread. #paxlovid #protease #inhibitors #ivermectin #COVID19
Dr. John Campbell: Paxlovid vs Ivermectin
New Pfizer antiviral and ivermectin, a pharmacodynamic analysis. Paxlovid (PF-07321332), C₂₃H₃₂F₃N₅O₄
Paxlovid is designed to block the activity of the SARS-CoV-2-3CL protease, an enzyme that the coronavirus needs to replicate. Co-administration with a low dose of
Nov 9, 2021 6 tweets 2 min read
February 15 and August 15, 2021 C19 Vaccination during Pregnancy & First-Trimester Miscarriage
compares to
March 2019 Norway study.

#percentage doesn't fit. #covid & #COVIDVaccination era has more miscarriages.

March 2019 Norway study: March 2019 norway study
file:///C:/Users/User/Downloads/Role_of_maternal_age_and_pregnancy_history_in_risk.pdf
Nov 8, 2021 6 tweets 2 min read
SARS-CoV-2 PL-Pro (Nsp3) Inhibitors.
#nature #plantbased
Salvia miltiorrhiza.

PLpro has an implication in viral invasion by shutting down the viral-induced host innate immune response. Dihydrotanshinone I appears to be the strongest inhibitor amongst the tanshinone ..1/n derivatives. It is a natural compound isolated from lipophilic fraction of Salvia miltiorrhiza, which has a long history in traditional Chinese medicine. Several derivatives of tanshinones were previously reported to be inhibitors for SARS-CoV-1 PLpro and to a lesser extent ..2/n
Nov 8, 2021 12 tweets 3 min read
SC2 MPro inhibitors alternatives, plant flavonoids.
1/n
#MPro #SARSCoV2 #inhibitor
Kaempferol, Quercetin, and Rutin
were able to bind at the substrate binding pocket of 3CLpro with high affinity and interact with the active site residues ..
chemrxiv.org/engage/chemrxi… such as His41 and Cys145 through hydrogen bonding and hydrophobic interactions. In fact, the binding affinity of Rutin was much higher than Chloroquine (1000 times) and Hydroxychloroquine (100 times) and was comparable to that of the reference drug Remdesivir. 2/n
Nov 8, 2021 8 tweets 4 min read
In silico identification of natural products from Centella asiatica as SARS-COV-2 main protease (M pro) inhibitor. 1/n

❌❌❌ #ritonavir #molnupiravir in #COVID19
No more #tryglyceride #mutagenesis

pubmed.ncbi.nlm.nih.gov/34345605/ 2/n
Nov 2, 2021 4 tweets 2 min read
The vaccinated🐶🐶developed autoantibodies to many of their own biochemicals,➡️the vac 🐶🐶 were attacking their own fibronectin, which is involved in tissue repair, cell multiplication & growth, etc.

Thanks @impressionist81 🙏
dogsnaturallymagazine.com/purdue-vaccina… The fact that vaccines and adjuvants can trigger a pathogenic autoimmune response is corroborated by animal models. The use of animal models has enabled the study of the effects of application of
pubmed.ncbi.nlm.nih.gov/27417999/
Nov 2, 2021 6 tweets 4 min read
Comparison of SARS-CoV-2 Receptors Expression in Primary Endothelial Cells and Retinoic Acid-Differentiated Human Neuronal Cells
#TMPRSS2 #NRP1 #ACE2 levels increase when cells are treated with #retinoicacid (RA).
#ACE2 and #TMPRSS2 expression in subsets of lung epithelial cells are regulated by androgen-signaling. #NRP1 is highly expressed in the respiratory and olfactory epithelium, which can explain the SC2 infectivity and spreading through both the lungs and olfactory bulb. NRP1 is involved in axonal guidance and pruning. Data indicate that
Nov 2, 2021 5 tweets 3 min read
Monoclonal Human Antibodies That Recognise the Exposed N and C Terminal Regions of the Often-Overlooked SARS-CoV-2
ORF3a Transmembrane Protein

#Orf3a #SARSCoV2
#mAbs
1/n
mdpi.com/1999-4915/13/1…
Nov 2, 2021 6 tweets 3 min read
High SARS-CoV-2 seroprevalence in persons experiencing homelessness and shelter workers from a day-shelter in São Paulo, Brazil
. 1/n

#covid in #homelessness ppl & #shelter #workers #Brazil

journals.plos.org/plosntds/artic… Serum samples, nasopharyngeal and oropharyngeal swabs of persons who are homeless and shelter workers collected from August 25th-27th, 2020 were tested for the presence of anti-SC2 IgM and IgG antibodies by ELISA and SARS-CoV-2 RNA by RT-qPCR, respectively. 2/n
Oct 16, 2021 7 tweets 3 min read
Potential Cause of COVID-19 ‘Long-haulers’ : antibodies specific for ACE2.

#ACE2
#LongCovid

"The antibody creates problems for the immune system by attacking the ACE2"

news.uams.edu/2021/09/09/uam… "Patients with ACE2 antibodies have lower activity of soluble ACE2 in plasma. Plasma from these patients also inhibits exogenous ACE2 activity. ...

journals.plos.org/plosone/articl…
Oct 1, 2021 5 tweets 3 min read
Ivermectin 0.2-0.4mg/kg works at the outer and inner part of cells, thru P2X, GlycineCls, has anti-inflammatory effects, proven for decades, etc.
Molnupiravir 800mg twice a day for 5 days, works thru the RdRp. Can this antiviral have the side effects similar to Remdesivir ? Comparison studies about Molnupiravir.
Gordon et al. : the high frequencies of G-to-A and C-to-U transition mutations in coronaviruses exposed to molnupiravir.

#host DNA #mutagenesis possibility

nature.com/articles/s4159…
Sep 29, 2021 5 tweets 3 min read
Variant that could potentially affect more people who are vaccinated against COVID-19.
#Vaccine creates #variants

#Nsp12 #W152L #C241U Mutations

Also found in all VOCs #P323L and #D614G mutations.

"What Is the R.1 COVID-19 Variant? What We Know So Far" health.com/condition/infe… Orf9c
Sep 22, 2021 7 tweets 2 min read
Five of nine C19 positive pregnant women, have same lesions in the placenta.

That’s 55,55%.

“These placentas showed lesions characterized by villous trophoblast necrosis with intervillous space collapse and..
nature.com/articles/s4137… variable amounts of mixed intervillous inflammatory infiltrate and perivillous fibrinoid deposition.”
Sep 3, 2021 6 tweets 1 min read
"Although it is rare with vaccination, the COVID-19 vaccine is likely the cause of hepatotoxicity in our patient based on a diagnosis of exclusion. In this

Cureus | Drug-Induced Liver Injury After COVID-19 Vaccine cureus.com/articles/63877… case, the patient had a cholestatic pattern with elevated ALP and bilirubin with mild elevation in the transaminases."

Based on population-based studies, drug-induced liver injury incidence varies between 13.9 and 19.1 cases per 100,000 people per year.

From the spontaneous
Aug 27, 2021 10 tweets 2 min read
Dr. Peter McCullough & Dr. Al Johnson
COVID questions. A 🧵.
The vaccines are failing against Delta Variant (DV).
All of us esp parents and teacher need to pay attention to the symptoms. Early treatment are the best, don't wait for the PCR. If a kid has symptoms, get him out of school, put him in his bedroom with windows open. Early test: antigen test. Kids should not be vaccinated, give them Vit C Vit D, Zn, polyphenol supplements e.g. Quercetin. Aspirin for fever. Check aapsonline.org/covidpatientgu…
Aug 27, 2021 9 tweets 2 min read
Lambda variant.
"Bamlanivimab FULLY LOST BINDING
toLambdavariant.
Structural modeling ofcomplexesofRBD-human receptor, ACE2, andBamlanivimab suggest the potentialbasisofthe
change of binding."
biorxiv.org/content/10.110… This could be the possible link of : mAbs can challenge the virus to mutate into a newer variant.

"Overall antibodies fromPfizer-BioNTech vaccinated individuals drop dramatically ( ~ 4.0times for lambda)after sixmonths."
Aug 11, 2021 7 tweets 2 min read
Polysorbate 80, also present in Moderna C19 vac.
PS80 delays the inhibition of thermal aggregation process of insulin. Addition of PS80 leads to an increase in beta-sheet structure and concomitant altered morphology and ...
#betaamyloid
#protein
#aggregation enhanced stability of the formed spherulites. This may impact safety and efficacy of the protein drugs product.
Aug 10, 2021 10 tweets 4 min read
The receptors involved in SARS-CoV-2 infection are co-expressed in the cells of the NVU, especially in astrocytes and microglial cells. These receptors are functionally active as exposure of endothelial cells to the SARS CoV-2 S1 protein subunit ..
#HIV
#ACE2
#brain altered the expression pattern of tight junction proteins, such as claudin-5 and ZO-1. Additionally, HIV-1 infection upregulated ACE2 and TMPRSS2 expression in brain astrocytes and microglia cells.
#astrocytes
#microglia
#brain