See short tweetorial for details:
#clearing #imaging #3D #deeplearning #AI @CellCellPress
Here are some shortcomings we know and want to improve:
a-Method cannot eliminate the high autofluorescence of human tissue.
b-There are still no light-sheet microscope systems to scan the human brain.
d-The labeling and clearing process still takes months depending on the size of organs. We need to find ways to get this down to some days level.
f-The best human organ mapping would require very fresh organs e.g., shortly after death, which is very difficult to access.
, Fritz Thyssen Stiftung, @NIH, @ERC_Research.