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An eerie video of a moving Enterobius vermicularis worm, shared by Sabir Hanif Hanif
#Fortheloveofmicrobiology #clinicalmicrobiology
#mmidsp #microrounds #IDpath #ASMClinMicro
#MicroTwitter #WomeninMicrobiology #STEM #medtwitter #ClinMicro #microbiologypakistan #PathBugs twitter.com/i/web/status/1…
#Fortheloveofmicrobiology #clinicalmicrobiology
#mmidsp #microrounds #IDpath #ASMClinMicro
#MicroTwitter #WomeninMicrobiology #STEM #medtwitter #ClinMicro #microbiologypakistan #PathBugs twitter.com/i/web/status/1…
Enterobius vermicularis, also known as pinworm, is a common intestinal parasite that infects humans. These worms are small, about 1 cm, are white or translucent in color. They are easily visible to the naked eye and can sometimes be seen moving around the anal area.
The video shows a moving Enterobius vermicularis worm with its distinctive cylindrical shape and tapered end. The worm moves by contracting and relaxing its muscles in a wave-like motion, allowing it to crawl or wriggle forward.
Wet mount and gram stain showing free hooklets of Echinococcus spp on cyst fluid from a 75 yr old F. Circled the hooklets in following pictures.
#Fortheloveofmicrobiology #clinicalmicrobiology #microrounds #IDpath #ASMClinMicro
#MicroTwitter #ClinMicro #microbiologypakistan
#Fortheloveofmicrobiology #clinicalmicrobiology #microrounds #IDpath #ASMClinMicro
#MicroTwitter #ClinMicro #microbiologypakistan
Echinococcosis or hydatid disease is caused by the larval stage of the dog tapeworm, Echinococcus granulosus. The definitive host for this disease is the dog or other canids and the intermediate hosts are cattle,sheep,pigs,goats or camels. Man is an accidental intermediate host
Hydatid disease in humans is potentially dangerous depending on the location of the cyst. Some cysts may remain undetected for many years until they become large enough to affect other organs. Symptoms are then of a space occupying lesion.
Candida albicans on chocolate agar, with starry projections
#Fortheloveofmicrobiology #clinicalmicrobiology #mmidsp #microrounds #IDpath #ASMClinMicro
#MicroTwitter #WomenInSTEM #WomeninMicrobiology #STEM #medtwitter #ClinMicro #microbiologypakistan #PathBugs
#Fortheloveofmicrobiology #clinicalmicrobiology #mmidsp #microrounds #IDpath #ASMClinMicro
#MicroTwitter #WomenInSTEM #WomeninMicrobiology #STEM #medtwitter #ClinMicro #microbiologypakistan #PathBugs
Yeast will grow on bacteriological media (sheep blood agar and chocolate agar).
They may appear as small, creamy or white colonies that are somewhat more raised than staphylococcal colonies.
They may appear as small, creamy or white colonies that are somewhat more raised than staphylococcal colonies.
A presumptive identification of C albicans can be made by observing pasty,yellow-white colonies from which colony projections, often referred to as "feet," extend out from the margins.
These feet have typically been considered a characteristic of C albicans
These feet have typically been considered a characteristic of C albicans
India ink preparation of CSF from a patient with cryptococcal meningitis showing the budding yeast cells of C. neoformans surrounded by a characteristic wide gelatinous capsule. The yeasts also show narrow-base budding and characteristic variation in size.
The space occupied by the capsule shows as a clear space between the gray background of the ink particles and the refractile edge of the cell.
India ink/nigrosin stain is a negative, acid stain. This means that the dye easily gives up a hydrogen ion (proton) and the chromophore of the dye becomes negatively charged. Since the surface of most organims cells is negatively charged, the cell surface repels the dye.
CSF received from a 12 year old child with Ewing sarcoma
India ink preparation showing rounded yeast cells surrounded by a halo, highly suggestive of Cryptococcus neoformans
#Fortheloveofmicrobiology #clinicalmicrobiology #mmidsp #microrounds #ASMClinMicro #microbiologypakistan
India ink preparation showing rounded yeast cells surrounded by a halo, highly suggestive of Cryptococcus neoformans
#Fortheloveofmicrobiology #clinicalmicrobiology #mmidsp #microrounds #ASMClinMicro #microbiologypakistan
India ink stain, previously known as the Nigrosin stain, is a quick, low-resource method. It is widely used for the microscopic detection of cryptococci in CSF. Its a negative stain used to determine the organism’s cellular morphology.
In the India ink preparation, stain fills the background and the thick polysaccharide capsule does not take up the stain, resulting in an appearance of a refractile large white circle against a dark background, giving the appearance of a halo of light
Many times when susceptibility testing is done for P. aeroginosa, a scenario similar to shown in the picture is encountered.
We see that TZP has produced a D shape on the IPM side. This might look very similar to the phenomenon seen in gram positive ICR strains. So what is it?
We see that TZP has produced a D shape on the IPM side. This might look very similar to the phenomenon seen in gram positive ICR strains. So what is it?
Published data says that this result is most likely due to
inducible expression of the P. aeruginosa AmpC beta-lactamase.
inducible expression of the P. aeruginosa AmpC beta-lactamase.
Certain enteric
(Serratia, Providencia, Citrobacter, Enterobacter, Morganella) and non-enteric organisms (P. aeruginosa, Aeromonas) can up-regulate expression of
their chromosomally-encoded ampC genes in response to sub-inhbitory concentrations of certain
b-lactam antibiotics
(Serratia, Providencia, Citrobacter, Enterobacter, Morganella) and non-enteric organisms (P. aeruginosa, Aeromonas) can up-regulate expression of
their chromosomally-encoded ampC genes in response to sub-inhbitory concentrations of certain
b-lactam antibiotics
1/ Happy Friday!
Today I am celebrating #scicomm! As of this week, I have contributed 21 articles to the @ASMicrobiology Bugs & Drugs blog! Here's a recap of some of these articles for those who love clinical #microbiology
#IDTwitter @ASCLS @CUAnschutz #MedEd @JClinMicro
Today I am celebrating #scicomm! As of this week, I have contributed 21 articles to the @ASMicrobiology Bugs & Drugs blog! Here's a recap of some of these articles for those who love clinical #microbiology
#IDTwitter @ASCLS @CUAnschutz #MedEd @JClinMicro
2/ First, just a quick note on writing for @ASMicrobiology. Volunteering my time to write has been one of the highlights of the last 2 yrs for me! So grateful for the amazing Bugs & Drugs team, especially @JulieMarieWolf @JClinMicro @ScienceInTheDMV who took a chance on me.
3/
YELLOW FEVER AND FRACTIONAL DOSES
#Publichealth piece discussing the yellow fever outbreak of 2015/16 in Africa & highlighting the amazing work of researchers working on fractional #vaccine dosing. It's an amazing story, full of impressive characters.
asm.org/Articles/2021/…
YELLOW FEVER AND FRACTIONAL DOSES
#Publichealth piece discussing the yellow fever outbreak of 2015/16 in Africa & highlighting the amazing work of researchers working on fractional #vaccine dosing. It's an amazing story, full of impressive characters.
asm.org/Articles/2021/…
Today's talk now posted for my FAVOURITE topic:
How to study & learn clinical/medical #microbiology.
🧠What to know
🧠How we learn
🧠Free resources
🧠Motivation
🧵below & slides available free: tinyURL.com/LearningMicro
#IDTwitter #MicroMedEd #ASMCPEP #clinmicro #ASMClinMicro
How to study & learn clinical/medical #microbiology.
🧠What to know
🧠How we learn
🧠Free resources
🧠Motivation
🧵below & slides available free: tinyURL.com/LearningMicro
#IDTwitter #MicroMedEd #ASMCPEP #clinmicro #ASMClinMicro
This talk expands on content from my ASM post from June.
asm.org/Articles/2021/…
... which outlined traits of experts & how we can use them to learn (eg. with visual tools):
👉These slides (summarized next) put this in the context of preparing for clin/med micro/ID exams.
2/14
asm.org/Articles/2021/…
... which outlined traits of experts & how we can use them to learn (eg. with visual tools):
👉These slides (summarized next) put this in the context of preparing for clin/med micro/ID exams.
2/14
🧠WHAT DO YOU NEED TO KNOW?
Your exam will have a syllabus & information about the exam format itself. These are key resources. But remember that you're preparing for both the exam and your career beyond that.
3/
Your exam will have a syllabus & information about the exam format itself. These are key resources. But remember that you're preparing for both the exam and your career beyond that.
3/
1/I want to take some time to share findings from our semi-recent PETACS study. This was a wonderful collaboration between @COpedsID faculty, survey designers, and #microbiologists across the US.
@JClinMicro @ASMicrobiology #ASMClinMicro
jcm.asm.org/content/59/3/e…
@JClinMicro @ASMicrobiology #ASMClinMicro
jcm.asm.org/content/59/3/e…
2/ First, I want to emphasize that this project highlights many of the reasons I chose to pursue a #PhD in clinical & translational science. As a long-time microbiologist, I can say that the clinical lab doesn't often have a seat at the clinical effectiveness / research table.
3/ This project focuses on the tracheal aspirate culture- a diagnostic process that is greatly limited by contamination with normal respiratory flora, and which lacks consensus or standardization across labs and hospitals. @ASCLS @ASMicrobiology @ASCP_Chicago
#MicroRounds (Day 733): Hypothetical case: a parent hears their child use the toilet but not flush (typical behavior!).
But upon entering the bathroom they're horrified to see this worm in the bowl with their kid's poop! How freaked out should they be?? #ASMClinMicro #IDTwitter
But upon entering the bathroom they're horrified to see this worm in the bowl with their kid's poop! How freaked out should they be?? #ASMClinMicro #IDTwitter
Cont.: Here's some additional information (from your friendly neighborhood parasitology lab).
1) Yes, it's actually a worm.
2) You zoom in close and see this on one of the ends of the worm...
1) Yes, it's actually a worm.
2) You zoom in close and see this on one of the ends of the worm...
#MicroRounds: If you see this worm in your toilet: relax. It didn't come from a person!
A horsehair worm. Nematomorpha (i.e. "looks like a nematode") is a phylum that infects grasshoppers, crickets & drives them to water. A common false human parasite #ASMClinMicro #IDTwitter
A horsehair worm. Nematomorpha (i.e. "looks like a nematode") is a phylum that infects grasshoppers, crickets & drives them to water. A common false human parasite #ASMClinMicro #IDTwitter
Ok, sort of weird to say...but today I'm dedicating some time to SPIT (formally known as #Saliva). It's the super hot topic this week in the ever-changing #COVID19 diagnostic landscape we are living in, so let's take a quick peek at some of the evidence out there (thread)
I completely understand the importance of testing saliva, particularly as it relates to lack of supplies and resources. It can be a feasible option that basically eliminates HCW risk of infxn during collection. It can also save supplies: no swabs, media, possibly fewer reagents.
First paper is here: DOI: 10.1128/JCM.01659-20
-53 paired samples from OP and/or NP swabs, and saliva were collected
-Standard RNA extraction methods were used for the swab samples, a quick extraction method was used for saliva. Handling high-viscosity samples is discussed.
-53 paired samples from OP and/or NP swabs, and saliva were collected
-Standard RNA extraction methods were used for the swab samples, a quick extraction method was used for saliva. Handling high-viscosity samples is discussed.
Antibiotic testing. Let's go.
Tentatively calling this #tweetorial "Antibiotic testing: why and how and huh?"
Important caveat: this is a HUGE topic. I’m mostly going to focus on 1) basic rationale and 2) laboratory methods. And 3) try not to say anything wrong/misleading!
Tentatively calling this #tweetorial "Antibiotic testing: why and how and huh?"
Important caveat: this is a HUGE topic. I’m mostly going to focus on 1) basic rationale and 2) laboratory methods. And 3) try not to say anything wrong/misleading!
Question #1: Why would a person want to know if bacteria A is resistant to antibiotic B?
And the answer is ...
All are legit answers! But it's very situation dependent and you'd have different reasons for each. Let’s go through them.
#tweetorial #pathtweet #asmclinmicro
All are legit answers! But it's very situation dependent and you'd have different reasons for each. Let’s go through them.
#tweetorial #pathtweet #asmclinmicro
