Thread by @felixjhartmann

Out today! New tool for everyone interested in immune monitoring using mass cytometry (#CyTOF). Provides a comprehensive reference panel to identify major immune cell lineages, functional subsets and checkpoint molecule expression.

Some highlights below:

cell.com/cell-reports/f…

No more spending several months to establish a panel! Our workflow is ready-to-go, consists of
commercially available reagents (@fluidigm) and is already titrated. Using this backbone, >98% of peripheral blood cells can be clearly identified by positivity for >4 antigens

More:

We further validated our approach by flow cytometry and across various research centers. Also, works comparable on both live and pre-fixed cells. Did we miss your favorite marker or you have a specific hypothesis? Can easily be extended with 10+ surface and intracellular markers

Using this framework, we performed comprehensive monitoring of immune reconstitution upon bone marrow transplantation in cancer patients and could identify cellular signatures associated with the development of #GvHD in a subset of patients.

Finally, thanks a lot to all co-authors, it was a pleasure @StanfordMed @stanfordimmuno @parkerici @Spitzerlab @Bendall_Lab @CellReports. And watch this space for more to come soon! 😀