Thread by @MusiciScience

1/50 #Science is an ongoing process that serves to investigate phenomena, gain new insights or "correct prior knowledge" and this only works in #discourse. This includes diversity of #Opinion, #tolerance and a broad debate space. This is a large thread.

2/50 "#trust the science" is in my view the most #antiscience statement ever. #Questioning science is how you do #Science. This thread gives some examples and further questions.
scientificamerican.com/article/why-do…

3/50 What about good ##scientific #Practice? I found a glaring example here (of all things, in what I consider to be a rush to #vaccine #approval). #blotgate
trialsitenews.com/a/startling-ev…

4/50 What about #false #positive tests?
False-positive diagnosis caused by COVID-vaccine has already been reported (#asymptomatic researchers who handled noninfectious SARS-CoV-2 nucleic acids and subsequently tested pos. on #surveillance screening).
nytimes.com/2020/11/12/hea…

5/50 #False Covid-19 #cases due to #contamination by inactivated virus #vaccine:
science.org/doi/10.1126/sc…

6/50 The best policies and practices for preventing laboratory #contamination should take place before initiating research: Once a space is contaminated with DNA, it is very difficult to #decontaminate.
L. R. Robinson, McCarthy et al., OSF Preprints, 10.31219/osf.io/9svjq (2020)

7/50 What actually happens when a #Covid #syringe falls and breaks?
#Ocular #surface #erosion after suspected exposure to #evaporated COVID-19 vaccine
ncbi.nlm.nih.gov/pmc/articles/P…

8/50 Another question: What is #gene #therapy? In the broadest sense, gene therapy is the use of #genetic material in the treatment or prevention of disease...

9/50 ...In the FAQs, the #ASGCT explicitly explains that the introduced "genetic material alters how a single #protein or group of proteins is produced by the cell." This is a provision under which #mRNA #techniques also fall.
asgct.org/education/more…

10/50 There was an interesting #debate that was published via the Berliner Zeitung (concept of #gene #therapy). Gene therapy is always present when stable expression of a gene product (RNA or protein) occurs due to a nucleic acid sequence present outside the cellular #genome.

11/50 With the exception of Valneva and Nuvaxovid, all corona #vaccines available in Germany fulfill exactly this condition; they can therefore without doubt be described as gene #therapeutics, even if they do not normally alter the #chromosomal #genome.
berliner-zeitung.de/coronavirus/zu…

12/50 But what about the criterion of #genome #modification and the quality of the #vial content of the #Moderna and #Pfizer monovalent & bivalent gene therapies?

13/50 Since February 2023, @Kevin_McKernan expert in #Genomics and #sequencing (e.g. can a novel #PCR method be used to enable the sequencing of hundreds of genes?) is engaged in...
nature.com/articles/nbt.2…

14/50 ...independent and ongoing analysis of #vial content of the #Moderna and #Pfizer. The following tweets are related to this letter:
veryslowthinking.substack.com/p/letter-rna-a…

15/50 Notable, #McKernan RNA and DNA #contamination that was again out of specification. He has used 9 different tests (assays) to characterise the contamination.
anandamide.substack.com/p/pfizer-and-m…

16/50 All gene therapies contain RNA that is out of spec (both longer and fragments). That DNA #contamination could be detected comprising "expression #vector #plasmids", which are remnants of the gene therapy #manufacturing #process...
anandamide.substack.com/p/dna-contamin…

17/50 ...and are #genetically active i.e. they are "#replication competent" in mammalian and #bacterial cells, capable of producing copies of themselves in vivo, with unknown but hypothetical effects.

https://twitter.com/Kevin_McKernan/status/1626056037164883968?t=23KaCwEnDXXvDjQ3DZqgMA&s=19

18/50 The amount of this #contamination is "orders of magnitude" above specific limits set by the #EMA ("dsDNA contamination levels are 100 fold higher and imply trillions of DNA molecules per #dose.").

https://twitter.com/Kevin_McKernan/status/1634511377397153792?t=ffWpkqmM13wYU1_z34OeTA&s=19

19/50 The #EMA had a prior appreciation of the need to set limits on DNA #contamination, although why and how those limits were set for these products is not stated in #documentation @sasha_latypova or @Kevin_McKernan have seen.

20/50 This demonstrates regulatory #concern about such #contamination that precedes product roll out.

https://twitter.com/Kevin_McKernan/status/1641909470493016070?t=Pcu0x2_jIUAIbP4QUK9enA&s=19

21/50 These #plasmids are made of double strand DNA (#dsDNA). #Injecting dsDNA into humans (in a DNA #vaccine, for example) is known to produce a human immune Type 1 #Interferon response via #STING.
pubmed.ncbi.nlm.nih.gov/35529875/

22/50 This means that regardless of why the #contamination is there, some form of undocumented #immune #response could be triggered in patients by the #dsDNA alone.
anandamide.substack.com/p/rapid-boil-p…

23/50 The plasmids have been engineered with specific characteristics including coding for #spike #protein and #antibiotic #Resistance to #neomycin and #kanamycin.

https://twitter.com/Kevin_McKernan/status/1626056038674755585?t=p5n4kmjl-js3MqzFnJVfsA&s=19

24/50 The #plasmids contain #genetic #code for #SV40 promoter, with some being engineered with that code occurring twice. SV40 #promoter makes the plasmid more capable of #gene expression...

...i.e. producing #proteins that have some effect, and doubling #SV40 increases gene expression capability further.
ncbi.nlm.nih.gov/pmc/articles/P…
To continue reading, please click on the next thread starting with the number 26.

Here is the link to the next thread (26-50/50):

https://twitter.com/MusiciScience/status/1642434294743179264?t=P1VmqqOIhwRyXCWgn0qyoQ&s=19

pubmed.ncbi.nlm.nih.gov/34498683/

Another source reference for tweet 4 and 5: Kelvin Kai-Wang To et al. Clin Infect Dis. 2022. [...] Two individuals whose respiratory specimens were contaminated by an inactivated SARS-CoV-2 vaccine strain (CoronaVac), likely at vaccination premises.
ncbi.nlm.nih.gov/pmc/articles/P…