Discover and read the best of Twitter Threads about #PCR

Most recents (24)

This is a concise, easy to understand explanation of the problems there are with the PCR Test for #Coronavirus

Based on this article:
bit.ly/2XLrazf

From this video:


#COVID19 #lockdownMelbourne #PCR #Masks #coronatest #BillGates
Dr. Andrew Kaufman also explains the PCR test . .

But regardless of what the PCR test actually tests for, what difference does it make, if you're going to class people as having tested positive, when they haven't even had the test!

Read 9 tweets
PCR

Pemeriksaan PCR merupakan golden standar pemeriksaan yg disampaikan WHO (maaf bagi penganut teori Konspirasi abaikan saja).
Dengan pemeriksaan PCR dapat diperiksa RNA virus nya dan bisa terdeteksi load virusnya. Dg pemeriksaan PCR dapat dikonfirmasi apakah terinfeksi Covid-19 atau bukan.
Dengan banyaknya akses utk bisa pemeriksaan PCR akan lbh sistematis apa yg akan dilakukan dlm pengendalian pandemi Covid-19. Jangan takut dg banyak pemeriksaan akan banyak diketahui konfirmasi positif.
Read 7 tweets
Thread über die Aussagekraft von #Covid19-#PCR-Tests und das #Containment bei der Kontaktnachverfolgung in Österreich.

Im #Ö1-Morgenjournal erklärt Benka (Gesundheitsministerium), warum Kategorie-I-Kontaktpersonen trotz negativem PCR-Test in Quarantäne bleiben müssen. 1/16
Eine Verkürzung der Quarantäne auf 10 Tage ggf. mit 2. Test wird angedacht.

Benka erklärt, dass die Vorgaben für Quarantäne nur Richtwerte wären und die Gesundheitsbehörden den Personenkreis ausweiten könnten. 2/16

Online bis 21.7.2020:
oe1.orf.at/player/2020071…
Die Falldefinition für Hochrisikontakte in Räumen wurde allerdings ab 15.5.2020 aufgeweicht und berücksichtigt den Infektionsweg über Aerosole nicht mehr.

Das #BMSGPK muss nachschärfen, sonst beruft man sich auf die "strengen Vorgaben des Bundes". 3/16

Read 19 tweets
Guys, there different methods of calculating Put Call Ratio (#PCR).
There are two types of PCR – 1. PCR OI 2. PCR Volumes.

PCR OI = Total Put OI / Total Call OI

Few traders prefers to calculate PCR for particular expiry, while few calculate it using combined data.

(1/n)
In combined data, one take all expiry and all strikes to calculate PCR. This can be easily done in excel from Bhavcopy data.

Many software/apps/websites provides PCR OI (all expiry) and some gives us an option to select particular expiry as well.

(2/n)
Now, when we calculate PCR-OI on monthly expiry day, we exclude the series which expired and calculate using data of coming expiries only. Some software do calculate in the same way.

(3/n)
Read 7 tweets
The Corona Simulation Machine: Why the Inventor of The “#CoronaTest” Would Have Warned Us Not To Use It To Detect A Virus

#KaryMullis, Inventor of #PolymeraseChainReaction #PCR

uncoverdc.com/2020/04/07/was…
#COVID19 #PCR-tests zijn wetenschappelijk zinloos

#WalterLippmann, de tweevoudig winnaar van de #Pulitzerprijs en misschien wel de meest invloedrijke journalist van de 20e eeuw, zei: 

“Waar iedereen hetzelfde denkt, denkt niemand veel.”

indignatie.nl/2020/06/27/cov…

#coronahoax
Read 3 tweets
#GEORGEFLOYD 24 Unanswered Q's

1. Who's REALLY the independent examiner? #michaelbaden why aren't you running a Tox Report? It's standard procedure. 2. Why did 2 @Hennepin commissioners try to remove #andrewbaker, stating he caused 'irreparable damage to #blackcommunity?

1/
3. Why did #EricGarner also say #Icantbreathe 11X & was also repped by Benji & Baden? 4. What did #DerekChauvin do exactly for #ENR for 17 years? 5. Where did #GeorgeFloyd sister get that tee-shirt? 6. Are print shops considered essential or was it a tasteful gift?

2/
7. Why was the casket too small? 8. Why does #GeorgeFloyd get 3+ memorials when my mom couldn't travel to bury her sister; while others have had to stand 6 feet apart like they're posing for a Soprano poster? While others have to attend virtual funerals via ZOOM?

3/
Read 10 tweets
[LIVE] Are people with #HIV and #TB more likely to die of #COVID19SA? Our #BhekisisaWebinar in partnership with @Auruminstitute is on NOW. We'll be live-tweeting the event, follow on for live updates. bit.ly/2UsZbmn
First up, Mary-Ann Davies, public health medicine expert at @WesternCapeGov health department will release SA's first preliminary data on the #COVID19SA death risk for #HIV and #TB patients from the Western Cape. #BhekisisaWebinar
Until now, there hasn't been much data on #TB and #HIV and how those conditions affect your risk of dying from #COVID19SA, Davies says. Getting good data is not easy. #BhekisisaWebinar bit.ly/2UsZbmn
Read 43 tweets
1) Der super saubere #Sars_Cov_2-#PCR-Test von #Drosten, kann auf das Erkältungsvirus #Coronavirus #CoVOC43 kreuzreagieren. Die Möglichkeit von falsch-positiven Tests deshalb, ist wochenlang bekannt. #CoVOC43 kommt auch bei Tieren vor.

#TestetdieTests

instand-ev.de/System/rv-file…
2) Gerade hier wird die ganze Glaubwürdigkeit von bestimmten Experten und sogenannten "#Faktenchecker|n" überdeutlich. Dass keine falsch-positiven Fälle bekannt sind, heisst nicht, dass es keine gibt, bei >95/98% Spezifität.
correctiv.org/faktencheck/me…
3) Bei einer #Sars_CoV_2-Prävalenz(Infektionsdichte) von ca.1% o. darunter, wie zeitweise vermutet, ist das genauso, als wenn man mit einem guten Fernglas (PCR-Test) versucht, im dichten Nebel, scharfe Umrisse (Viren) zu erkennen. Es funktioniert nicht!
Read 6 tweets
Nigeria has made a lot of progress in preparing and responding to disease outbreaks and epidemics. I remember my early days of public health; some 15yrs ago it's @WHONigeria that prepared and shared the #weeklyepidemiological report for Nigeria... a thread!
1/20
#Clap4NCDC
For every outbreak or public health emergency in Nigeria, we heard more about @WHO who appeared to be at the forefront with @Fmohnigeria and its agencies; old #NPI which merged with @NphcdaNG fought #polio with the support of @WHO @UNICEF @Rotary etc.
#Clap4NCDC 2/20
With time the #Epidemiology unit of @Fmohnigeria grew in strength and capacity to lead the response. They also produced and sent out the weekly epidemiology reports for Nigeria, which hitherto was shared by @WHONigeria in collaboration with FMOH for sure. #Clap4NCDC
3/20
Read 23 tweets
Va una semana y hemos logrado un gran salón de twitter-clases. 2796 gracias!!! Ahora iniciemos este nuevo hilo, que busca que tomes acciones preventivas contra el #SARSCoV2, para cortar su transmisión, entendiendo con lenguaje sencillo aspectos de su estructura y cómo infecta 👇
Todos los virus son parásitos intracelulares obligados, es decir, requieren de una célula viva para perpetuarse. Los coronavirus también lo son y deben su llamativo nombre al hecho de tener una forma esférica, de la que sobresalen espículas que les dan su apariencia de corona 👇
El genoma de los virus es muy variado. Los hay de DNA o RNA, de cadena doble o sencilla, y de longitud variable. Para que te hagas a una idea, en una célula, los humanos tenemos 12.000 millones de nucleótidos en 46 cromosomas de DNA de cadena doble. Eso es bastante, verdad??? 👇
Read 10 tweets
Creé mi cuenta de Twitter hace 10 años y solo tengo UN seguidor, así que ¡todo es ganancia! Soy profesor de @udea y quiero utilizar este canal para enseñar y comunicar lo que hacemos en ciencia, usando un lenguaje sencillo.
Empecemos con el protocolo de detección de #COVID 👇
¡Estamos Felices! Luego de arduas horas de trabajo, científicos de la @udea (Medellín, Colombia) hemos diseñado y desarrollado un PROTOCOLO llamado COLOMBIA para la detección específica y sensible del #SARSCoV2.
Les queremos contar detalles en este #hilo👇
Han leído sobre #PCR, el método usado en detección de #SARSCoV2 y otros patógenos. Es una técnica que usamos con frecuencia en biología molecular. Replicamos muchas veces TARGETS específicos, para lograr que aumente su cantidad y así poderlos detectar 👇
Read 16 tweets
Mwanasayansi mwenye ubobezi wa kimataifa kwenye maswala ya vitendanishi na vipimo (Diagnostics), Dr. Ally Mahadhy, aliandika haya kwenye ukurasa wake wa facebook, ni vyema wadau humu wakayapata ili kuongeza uelewa @Udadisi
@MariaSTsehai @fatma_karume
#ElimikaWikiendi
Uzi 👇🏽
"Kutakucha tu!

Tukiwa tupo kwenye taharuki juu ya kipimo cha corona (covid-19) hapa nchini, baada kutoa majibu ya positive kwa mbuzi na papai, naomba nitoe maoni yangu kama mdau katika tansia hii ya vitendanishi vya maabara (diagnostics). ..." /1
Nianze kwa kutangaza maslahi yangu binafsi kwenye mada husika. Mimi ni mtafiti kwenye eneo hili la vitendanishi (Medical and clinical diagnostics). Tasnifu (thesis) yangu ya umahiri (MSc in Biotechnolgy) iljikita kwenye kutengeneza njia (method) ya kutambua kwa haraka na wepesi/2
Read 61 tweets
#Impfen #Impfzwang #Grundgesetz #Grundrechte #Widerstand2020 #Widerstand

2,5 Mio Patienten konnten nicht operiert werden, obwohl nötig.
Folge: nach Schätzungen 5.000 und bis zu 125.000 Patienten werden sterben oder sind schon tot infolge #Lockdown!
tichyseinblick.de/meinungen/stud…
#Impfen #Impfzwang #Grundgesetz #Grundrechte #Widerstand2020 #Widerstand

Gestern #Fake, heute Wahrheit: Der große #Corona-Lügen-Report
Selten wurden #FakeNews so schnell zur regierungsamtlichen Wahrheit wie in Zeiten von #Corona.
reitschuster.de/post/gestern-f…
Read 76 tweets
Una semana más, te traemos las últimas novedades científicas destacadas sobre la #COVID19 🦠

(sigue el hilo👇)

#Coronavirus #COVID__19 #COVIDー19
📈La mortalidad global del #coronavirus podría ser un 60% mayor que la anunciada, según un estudio que analizó estadísticas de mortalidad en 14 de los países más afectados

➡ Primer estudio serológico en el estado de Nueva York: casi el 14% de personas dio positivo

#COVID__19
Hombres♂ y mujeres♀ parecen infectarse igual pero ellos corren un riesgo 2,5 veces mayor de síntomas graves, según un análisis en China

👉Otro estudio indica: buena ventilación, espacios abiertos y desinfección de baños, eficaces para limitar el virus en aerosoles

#COVID__19
Read 7 tweets
[LT] A 15 h retrouvez les jeudis de @Fr_Assos_Sante. Aujourd'hui "Se préparer au déconfinement et anticiper". Pour suivre cet événement en direct : tinyurl.com/ybso7kzh
Le webinair commence avec Claude Rambaud Vice-présidente et Jean Pierre Thierry, conseiller médical @Fr_Assos_Sante, Catherine Hill, épidémiologiste et biostatisticienne, Vincent Maréchal virologue, Sorbonne Université. Plus de 400 personnes déjà en ligne !
Les questions de stratégies de dépistage, d'isolement et de traçage seront abordées
Read 50 tweets
1.Vamos a por un nuevo hilo a desmontar una de las supuestas panaceas... Los #Test del #Covid_19 Todos quieren test. Parece que con ellos hecho todos serán felices y será la panacea para combatir el virus. Son ya hasta arma política los test. Sin embargo tienen sus limitaciones
2. En España tenemos dos pruebas diagnósticas. #PCR que detecta el ARN viral directamente. Y #Test los llamados rápidos que detectan los anticuerpos de defensa frente precoces IgM, o tardíos IgG. No tememos tets de antígenos para #Covid_19. La #PCR es de ámbito hospitalario
3. Los #Test rápidos de anticuerpos que tenemos sí se pueden hacer en cualquier sitios. Son formato tipo test de embarazo. Y OH sorpresa! No detectactan la enfermedad hasta que no tiene entre 7 y 21 días de evolución. Entonces no detectan ni asintomáticos ni la enfermedad precoz
Read 15 tweets
Senta que lá vem história:
Qual a razão numa pandemia como esta de #COVID19 causada pelo novo #coronavirus para se fazer testes em massa? Siga o fio... 🧗‍♀️🧗‍♂️
A primeira razão p/ ter em larga escala o teste da #PCR (do Inglês polymerase chain reaction test) é poder confirmar de imediato o diagnóstico de portadores de #COVID-19 É determinar quarentena, isolamento social e uma melhor observação evolutiva tanto do doente dos contactantes!
A outra razão importante sobre o #PCR é poder avaliar o material genético do #SARSCoV2 que está naquele momento circulando na comunidade e avaliar por exemplo a existência de mutações na região e/ou país.
Read 6 tweets
Thoughts on upcoming #COVID19 #serology tests:
This is actually quite a challenge! (#Diagnostics often involve a lot of complexities). There is a lot of pressure to roll these tests out, but they need to perform well, or we do more harm than good. #MicroRounds (A thread)
WHY we need these soon:
1. Contact tracing.
2. Can be used to test if a vaccine is working during a clinical trial (70 of them ongoing right now, I believe)
3. Inform public policy makers about rate of asymptomatic cases + previous infections/exposures =informed decision making
How is developing a #PCR different than developing a #serology test?
1. #PCR tests detect viral RNA/DNA (in this case RNA) and can be pretty straight-forward in terms of development
2. #Serology relies on knowing about the #SARSCoV2 structure and how the human body responds.
Read 13 tweets
Dermatologist are seeing more and more #skin symptoms related to #COVID19 . Acral lesions (i.e. hands and feet) are particularly frequent, specially in #children and #youngadult with no or few COVID symptoms. After seeing many cases, here is my vision: #Thread 👇
I think that these kind of lesions can be classified in four types. 1. Chilblain-like lesions, which are the most described, usually affecting many fingers / toes, either symmetrically or not. Pruritic and/or painful.
2. Insect-bite like lesions, that may also appear in the palm and the soles. Very #itchy and persistent (more than 2 weeks in some cases)
Read 10 tweets
Brilliant talk by Phill James @Grounded_circus on #SARSCoV2 genome sequencing using @nanopore sequencing. Giving humanity hope in these dark times. Here I will share some information from the talk. Thread.👇
Why #Nanopore for Sars-Cov2 genome sequencing? @nanopore sequencing allows us to do real-time sequencing cost-effectively and with low instrument cost. In the #COVID19 context, it can help us monitor the #coronavirus spread and vaccine efficacy.
.@nanopore was used before for monitoring other viral outbreaks, such as #Ebola: nature.com/articles/natur… and #Zika: genomemedicine.biomedcentral.com/articles/10.11…
Read 11 tweets
Sobre el pleito de las pruebas vs las no pruebas para #COVID19 y algunos de los atavismos de la medicina mexicana, abro el siguiente #Hilo:
1.- En 1988, México comenzaba a conocer la etiología (la causa) de las hepatitis virales.
En ese tiempo existían la Hepatitis A, la Hepatitis B y la llamada “no-A no-B” (en serio).
2.- La clasificación se hacía por historia clínica, signos y síntomas. Si estabas amarillo y habías comido en la calle era hepatitis A, si habías tenido una transfusión, era hepatitis B. El problema es que la mayoría de las hepatitis no producen síntomas …
Read 20 tweets
Growth rate is key during a disease outbreak. The rate of growth in the the total confirmed #cases in #Canada seems to be showing signs of slowing and pulling away from the continued steep trend line seen, for example, in the United States (Green). #COVID19 #coronavirus 1/25
This is a +ve sign, but still very early to tell, esp. with an est. 1M+ #Canadians having just returned from travel, and #COVID-19 #testing only targeting >severe cases (due to rationing & backlog in lab testing), we don’t have a good grasp on the level of community spread. 2/25
The number of total cases is what we want to know, but the true number of these is not known. Not by us epidemiologists, nor by any other research, governmental or reporting institution. 3/25
Read 25 tweets
Reporting progress on #coronavirus Home Test from our teams internal email: Just wanted to give a brief update from the #molbio team as we build momentum towards delivering an at-home, user-friendly, rapid #COVID19 test...We are actually detecting Covid19 #RNA in solution now! 1/
... we got early proof today that we can do reactions from RNA (the genetics code of #SARSーCoVー2 not DNA so it’s more insidious). Our method (which amplified the RNA without a machine) is very tolerant of #saliva - (we still have to crack open the virus RNA for full proof) 2/
The parenthesis in the above tweets are my commentary and I also edited out jargon. But wanted to share our internal optimism. We now know, except for breaking the #virus open to expose its #RNA we should be able to do a #COVID19 test without a #PCR machine @yale and @Penn 3/
Read 11 tweets
Thinking about a low cost easy to manufacture home test kit for #Coronavirus. Outline: #Nasal_swab (Q-tip) w/ freeze dried reagents for isothermal #DNA amplification with #COVID19 primers. Colorimetric readout by #iOS or #Android #App w/ #geolocation & #HIPPA compliant reporting.
Read-out would be in minutes - timer in App - read by camera in your smart phone. No other machines. Links to telemedicine groups for positive results. Built in positive control - e.g. no color = improper test, green NEGATIVE, red POSITIVE.
I’ve done my best creating when faced by a challenge. Next gen sequencing when my son went to new born intensive care - #454 @iontorrent. Ultrasound on a chip @ButterflyNetInc when wanted to monitor my daughter’s kidneys.
Read 28 tweets

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