Discover and read the best of Twitter Threads about #Interferon

Most recents (23)

1/🧵 Long COVID Brain Science: 🧠

For ~100M suffering 🌎, let’s use data to combat myths & #Misinformation

COVID is biologically dangerous long after virus is gone

#LongCOVID affects our….

📍Olfactory & Limbic Systems
📍 Interferon Autoimmunity
📍PET scans
📍Astrocytes

How? Image
2/ First, Epidemiological comparisons…

FLU vs. COVID

Survivors of viral infxns like #Flu don’t complain of 200 long-term symptoms & ~90% ongoing mood disorders.

#LongCOVID pts do.

Flu kills 0.5M/year vs COVID has killed 10X that (5M) in just 2 years.

bit.ly/3mJTBK4
3/ Consider this:

A study of 150k COVID survivors, hospitalized & not.

COVID survivors had 10-15X ‼️higher risk of considering #suicide at 1 year than 11M control patients.

This is horrifying, yet people still deny #LongCOVID’s importance.

WHY?

bit.ly/3HnHZpB ImageImageImage
Read 27 tweets
New paper out! Our own David Zander has successfully published his thesis work in @cddpress’ Cell Death & Disease!

Just to highlight this: David is an MD but he worked his tail off pipetting this study! A big Thank You to @TRR179_2 for the stipend!

1/6

nature.com/articles/s4141…
So what is it about?
• Damage to our cells’ genome DNA can lead to #cancer if it goes unnoticed (cell not committing suicide).
• Vice versa, many chemotherapies (and radiotherapy!) are based on killing cells by inducing #DNADamage.

In his study, David used…
2/6
…live cell microscopy (@EssenBioScience) to monitor cell proliferation and death upon treatment with doxorubicin (chemotherapeutic inducing DNA breaks) or gamma-irradiation (γIR). As expected, both treatments kill cells ⬇️

3/6
Read 7 tweets
@EllingUlrich Danke für ihre großartige Präsentation! 👍 @EllingUlrich

Denken Sie, dass der Interferon Escape von Omicrion (BA.1) auch die höhere Hospitalisierungsraten bei Kindern unter 5y, die in UK beobachtet werden, erklären könnte?

assets.publishing.service.gov.uk/government/upl…

1/n

@EllingUlrich Ist aufgrund des Interferon Escapes auch mit einer relativ zu vorherigen VoCs gesehen, höheren Infektiosität für Kinder zu rechnen?

Wenn bei Kindern Schwere ⬆️ (Hospitalisierung⬆️) & Infektiosität ⬆️ sollte die Politik die NPI für Kinder verschärfen statt lockern.

2/n
@EllingUlrich Wenn Omicron (i.e. BA.1) #Interferon Escape zeigt und damit einen Teil der unspezifischen Immunantwort unterläuft könnte man spekulieren dass es auch zu mehr fällen von Long CoViD kommt

Ist dazu etwas bekannt?

Macht diese Überlegung für Sie Sinn? @EllingUlrich @neurostingl
3/n
Read 4 tweets
IRF3 rules #interferon induction, IRF7 is its „backup“, IRF9 reigns IFN signaling, IRF1 is antiviral, IRF2 its antagonist, IRF5 receives increasing attention.

But how important are they really relative to each other? We tested KOs side-by-side in A549 ⬇️
mdpi.com/2073-4409/10/1…
Let me highlight this is a first-author work by our team’s #labtech Sandra Wüst, who has pipetted this whole work, coordinated and supervised „her“ student Paulina, *and* prepared the figures for the manuscript! Sandra rulez 🤩 Let me also tag @Sandy_Niesik for quite some help!
So a million thanks to all above 👆– great work!

Scientific part of the Tweet: IRF3 and IRF9 are as important as we thought, the others… meow… At least in A549 cells– that’s the big limitation (do *not* ask us to repeat this KO craze in other cell lines 🙄).
Read 4 tweets
Super exciting and very laborious new study @berlinnovation (with @UKL_Leipzig and us @dkfz) on the question of why #children are so much less likely to develop severe #COVID19 than adults! Central point (experts may smile tiredly 😉): #interferon!
1/7

nature.com/articles/s4158…
Our colleagues in Berlin studied cells in nasal swabs from a total of >80 people (single cell RNA sequencing, #scRNASeq)– 42 children and 44 adults, half of each were healthy and half #SARSCoV2-infected. What we found was intriguing: already in…
2/7
... healthy children, the cells of the nasal mucosa (epithelial and immune cells) were already on heightened alert! I.e. they had a significantly higher expression of the sensor system for #virus infection (esp. MDA5 and RIG-I). @VladimirMagalh in our lab was able to show ...
3/7
Read 9 tweets
Wirklich super spannende und sehr aufwändige neue Studie @berlinnovation (zusammen mit @UKL_Leipzig und uns @DKFZ) zur Frage, weshalb #Kinder so viel seltener schwer an #COVID19 erkranken als Erwachsene! Zentraler Punkt (der Fachmann wird müde lächeln 😉): #Interferon!
1/7
Die Berliner Kollegen haben die Zellen im Nasenabstrich von insgesamt >80 Menschen untersucht (Einzelzell-RNA-Sequenzierung, #scRNASeq)– 42 Kinder und 44 Erwachsene, je die Hälfte gesund und die andere #SARSCoV2-infiziert. Was wir fanden war faszinierend: bereits bei…
2/7
… gesunden Kindern waren die Zellen der Nasenschleimhaut (Epithel- und Immunzellen) bereits in erhöhter Alarmbereitschaft! D.h. sie hatten ein deutlich ausgeprägteres Sensorsystem für #Virus-Infektion (v.a. MDA5 und RIG-I). @VladimirMagalh in unserem Labor konnte zeigen, …
3/7
Read 10 tweets
Our latest study to understand the dynamic regulation of #interferon (#IFN) and #SARSCoV2 infection.

🧵summarizing what we found and what needs to be done to understand the dynamic interplay of infection and #IFN regulation. @iScience_CP #COVID19

cell.com/iscience/fullt…
We infected human airway cells (Calu3) with #SARSCoV2, and performed a time-series #RNAseq analysis to discover global transcriptional responses. We saw expression of #IFN (beta and lambda) and IFN stimulated genes in these cells.
Here is what #SARSCoV2 infection looks like in #Calu3 (human lung epithelial cells) cells.
Read 17 tweets
IMPORTANT THREAD on #COVIDTreatments - What Works & What Does not. Will be an ongoing threat.

Start with Dr Zarir Udwadia of Mumbai.
Link to Dr Udwadia's Video


1st Point: 90000+ Medical papers on Covid. UNFORTUNATELY, big divergence in Observational Studies impact and Real World impact of a rigorous RCT
RCT is Randomized controlled trial.
A Good website is Covid19Primer.com

2) What Works ?
(A) Steroids are essential. #DEXAMETHASONE 6mg/day for Critically ill Covid Patients. Reduces Death by 33/20% for Patients on Ventilators & Oxygen. ImageImageImage
Read 19 tweets
Congratulations, Agnieszka @BartenschlagLab! We actually began working on this during the last pandemic threat (#Zikavirus) and the way through editor and reviewer offices was not exactly aided by the current pandemic. But now it’s finally out! jvi.asm.org/content/early/…
Through TLR3, ZIKV induces proinflammatory #cytokines (IL-6, IL-10), which trigger STAT3 signaling, which in turn dampen #interferon responses. As #RIGI and #MDA5 sense ZIKV and induce IFN production, this antagonism eventually aids viral replication as we can show.
This is all so weirdly complex– a lot of work ahead to understand the details of innate immune regulation in virus infection. Ha, saying that– if you made it all the way to here in this thread, consider contributing to this special issue on exactly this topic ⬇️
Read 4 tweets
Reducing #COVID19 #Airborne #Transmission Risks on #PublicTransportation Buses
» doi.org/10.1101/2021.0…
Wearing of #facemasks reduced the overall particle count released into the bus by an average of 50%, the dispersion distance by several feet and #aerosol particles by 84.36%. Image
🔝#SARSCoV2 Infects Human Engineered Heart Tissues and Models COVID-19 Myocarditis
» doi.org/10.1016/j.jacb…
#COVID19 can kill heart muscle cells, interfere with contraction. Details of how #coronavirus infects #heart: models of tissue damage may help develop potential therapies. Image
High-content screening of coronavirus genes for innate immune suppression reveals enhanced potency of SARS-CoV-2 proteins
» doi.org/10.1101/2021.0…
196 protein products of seven coronaviruses: the genes encoded by #SARSCoV2 are generally more potent immune suppressors than others Image
Read 236 tweets
⚠️#Ocular #MRI Findings in Patients with Severe #COVID19: A Retrospective Multicenter Observational Study
» doi.org/10.1148/radiol…
All patients had nodules in the macular region, 8/9 (89%) had bilateral nodules, 2/9 (22%) had nodules outside the macular region. Screening needed. Image
⚠️🔝New-Onset #IgG #Autoantibodies in Hospitalized Patients with #COVID19
» doi.org/10.1101/2021.0…
An international team of researchers studying COVID-19 has made a startling and pivotal discovery: The virus appears to cause the body to make weapons to attack its own tissues. Image
⚠️🔝Patterns of myocardial injury in recovered troponin-positive COVID-19 patients assessed by cardiovascular magnetic resonance
» academic.oup.com/eurheartj/adva…
Damage to the heart found in more than half of #COVID19 patients discharged from hospital who show raised levels of troponin. Image
Read 235 tweets
With the .@NIAIDNews #ACCT1 trial final report published on 05 Nov 2020 and the .@WHO's #SolidarityTrial Preliminary report published on 02 December 2020, thought it would be good to take another look at both trials and find ways forward for the patients we are dealing with today
Motivated by a patient I am dealing with who will survive very severe #COVID19 in the setting of transplant, but her father, who was hospitalized earlier than her with moderate COVID, was left to progress and died before her discharge, given #remdesivir only after going on #BiPAP
Here is the link to the prior analysis and I think the new publications give some interesting details which will follow

Read 39 tweets
Pr que cela soit clair #Solidarity auquel #DisCoVeRy fournit les données de mortalité des patients a tiré au sort 1853 patients. La moitié ont eu l'hydroxychloroquine l'autre moitié rien.

Mortalité #HCQ : 11% (104/947)
Mortalité sans #HCQ : 9% (84/906)

medrxiv.org/content/10.110…
A tiré au sort 5451 patients entre remdesivir et pas de remdesivir

Mortalité #Remdesivir : 11% (301/2743)
Mortalité sans #Remdesivir : 11% (303/2708)
A tiré au sort 2771 patients entre lopinavir/ritonavir et pas de lopinavir/ritonavir

Mortalité #Lopinavir/ritonavir : 11% (301/2743)
Mortalité sans #Lopinavir/ritonavir : 11% (303/2708)
Read 4 tweets
🤧🤒 Erkältungssaison! Warum manche Viren sich nur zu bestimmten Jahreszeiten schlagartig verbreiten und ob das auch für #SARSCoV2 / #COVID19 eine Rolle spielt!

Hier ein kleiner Thread anlässlich des aktuellen Artikels von @Weber_Nina @derspiegel

spiegel.de/wissenschaft/m…

1/
„Saisonalität“ bei #Virusinfektionen ist extrem komplex. Eine hervorragende Übersicht bieten auch @VirusesImmunity (annualreviews.org/doi/10.1146/an…, hieraus einige Abbildungen im Thread!) oder auch @riffreporter ausführlich und auf deutsch (riffreporter.de/taktvoll/epide…).
2/
Hauptfaktoren für Saisonalität sind 1) Wetter, 2) Raumklima und 3) unser Sozialverhalten. Für jedes Virus gibt es eine optimale Kombination aus diesen Umgebungsfaktoren, unter denen es sich dann am effizientesten verbreitet. #Influenza Nov-Apr, „normale“ #Coronaviren Dez-Apr, 3/
Read 27 tweets
Time to share some thoughts and questions following the .@medrxivpreprint first release of results from the #SolidarityTrial that compared local standard care to #remdesivir, #HydroxyChloroquine #lopinavir_ritonavir and #interferon for treatment of patients with #COVID19
I have read the #solidarityTrial preprint: medrxiv.org/content/10.110…

Its supplementary material:medrxiv.org/content/10.110…

its posted protocol: who.int/publications/m…

The CRF: who.int/publications/i…

The final report from #ACTT1 for contrast: nejm.org/doi/full/10.10…
Everyone has focused on the results, which is understandable, yet understanding the specifics in #SolidarityTrial design compared to the #ACTT1 design, may bring light into the differences reported between trials and may give a way to do better in #COVID19 #therapeutics
Read 26 tweets
The preprints of the #SOLIDARITY trial are out on MedRxiv. While many may lament that all four drugs tested did not show benefit, this is a remarkable trial for many reasons. Thread
First, this was another #MultiArmMultiStage #MAMS study design; @MRCCTU made this highly efficient trial design globally acclaimed with #STAMPEDE. The more recent #RECOVERY trial was another example of this.
@MaxParmarMRCUCL @Prof_Nick_James @PeterHorby @MartinLandray
What the #MAMS design does is enable testing multiple drugs simultaneously, flexibility to drop unpromising ones & add new promising ones even midway during the trial. This was crucial in the #COVID__19 pandemic where the situation has been constantly evolving
Read 16 tweets
🔥Les résultat de #Solidarity @WHO, essai mère de #DisCoVeRy sont là

Confirme déception des antiviraux "repositionnés"

Nbre de patients impressionnant : 2750 Remdesivir, 954 Hydroxychloroquine, 1411 Lopinavir, 651 Interferon+Lopinavir, 1412 Interferon, and 4088 no study drug
405 hôpitaux, 30 pays, c'est ENORME !
On juge de la mortalité, un critère fort

#Hydroxychloroquine : va dans le sens d'augmentation de la mortalité mais NON SIGNIFICATIF donc on ne peut rien dire
#Remdesivir : AUCUNE DIFFERENCE DE MORTALITE, comme on le dit depuis des mois en analysant les différents essais
Read 10 tweets
Current list of widely available therapeutics for #Covid_19

Added Bromhexine, Nitazoxanide, & inhaled interferon

h/t @__ice9
cc @drakchaurasia @drkrvcvijay @DrJoyeeta @drprashant86jmd

Current list of widely avai...ImageImage
Commonly available drugs:
(minimum side effects)

Bromhexine+Omeprazole
Famotidine
Loperamide
VitaminsABCD+Zinc
Quercetin+Bromelain

Colchicine trial ongoing

Combine with 1 among these prescription drugs:
Doxycycline/Indomethacin/Dipyridamole/Ciclesonide

Commonly available drugs: (...
Loperamide is the odd one out here.

I put it because it is non-prescription & there is A research paper which suggests that it could disrupt the Cholesterol Synthesis Pathway

Prescription alternatives would be Fluvostatin + Losarton

Read 13 tweets
🔥Voilà un thread afin de ne pas avoir à répéter tout le temps la même chose sur la recherche médicale thérapeutique pendant #COVID19 #SARS_CoV_2 #pandemic

👇👇
1/ Les infectiologues cliniciens n'attendaient pas de ttt antiviral permettant une amélioration "spectaculaire" pr une maladie aiguë virale sévère (cf dengue, zika, chik, grippe), il était donc nécessaire d’évaluer rigoureusement et d’espérer un ttt un peu efficace et non toxique
2/ Il existe de nombreux candidats repositionnés qui ont été utilisés et testés et ce dès le début de la pandémie : #lopinavir/#ritonavir, #remdesivir, #hydroxychloroquine, #interferon ß pour n’en citer que quelques-uns
Read 35 tweets
A #practical topic for today. #Pregnancy changes things - but more so for our #neurology patients - or not? #pearls from the #EAN2020 #MedEd #MedTwitter #MedStudentTwitter #neurologyresident @EANeurology @WNGtweets
Let’s first address #multiplesclerosis. Patients get better in third trimester and 30% relapse #postpartum. Immune responses shifts to anti-inflammatory in 3rd trimester to accommodate fetus - #etcomehome - but rebounds after birth. #MedEd #doubledoc #AcademicTwitter
Continue #firstlineinjectables #glatiramer and #interferon - these don’t cause #teratogenicity. In flares, consider IVIG and PLEX. How about #steroids? Usually fine except in 1st trimester when they can cause birth defects #MedEd #MedStudentTwitter #MedTwitter
Read 6 tweets
1/
Should we use #Lopinavir-ritonavir (LPV/r) + #Ribavirin (RBV) +/- #Interferon beta-1b (IFN) to treat #SARSCoV2 #COVID19? Let’s take a look at the data.

Here’s: #HowIReadThisPaper on @TheLancet trial of triple therapy for COVID19

Hung et al: thelancet.com/journals/lance…
2/
First, let’s assess our baseline beliefs about triple therapy:

Before reading this study, when considering triple therapy as a treatment for patients with COVID19, I think the most important component is likely to be:
3/
Background: A 2003 case series suggested ↓mortality (vs historical controls) in SARS patients treated with LPV/r + RBV :

ncbi.nlm.nih.gov/pmc/articles/P…

In 2015, LPV/r and IFN led to ↓viral load and improved clinical outcomes in animal models of MERS:

pubmed.ncbi.nlm.nih.gov/26198719/
Read 30 tweets
1/8 Pharmacien Hospitalier clinicien en réanimation et chercheur à Amiens, Je vois nos capacités de soins de réanimation en extrême tension et une prise en charge du #COVID-19 sans consensus.
2/8 Afin de trouver la meilleure thérapeutique je souhaite apporter mon analyse. L’#hydroxychloroquine est cardiotoxique pour au moins 2-3 mois, ce qui risqué chez les personnes fragiles, justement sujettes aux formes sévères du COVID.
3/8 L’#azithromicine (sécréteur d’Interferon I) suffirait-elle ? Les chinois ont montré que l’#hydroxychloroquine n’apportait rien à de l'interféron α2b inhalé + antiviral subject.med.wanfangdata.com.cn/UpLoad/Files/2…
Read 8 tweets
Starting a series on clinical Pearls I am gathering in #COVID19 patients based on experiences of many experts

Will add as we go along

Feel free to add your own observations/experiences

#covidclinicalpearls /1
Anosmia is likely the most specific #COVID19 related symptom
30% of patients have anosmia as their 1st symptom
#covidclinicalpearls /2
Around 90% of patients have fever.
50% maybe afebrile at the time of presentation
Fever tends to be very resistant to routine measures in hospitalized patients
There is no consensus that NSAIDs are to be avoided in #COVID2019 patients

#covidclinicalpearls /3
Read 66 tweets

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