Discover and read the best of Twitter Threads about #saliva

Most recents (3)

Ok, sort of weird to say...but today I'm dedicating some time to SPIT (formally known as #Saliva). It's the super hot topic this week in the ever-changing #COVID19 diagnostic landscape we are living in, so let's take a quick peek at some of the evidence out there (thread)
I completely understand the importance of testing saliva, particularly as it relates to lack of supplies and resources. It can be a feasible option that basically eliminates HCW risk of infxn during collection. It can also save supplies: no swabs, media, possibly fewer reagents.
First paper is here: DOI: 10.1128/JCM.01659-20
-53 paired samples from OP and/or NP swabs, and saliva were collected
-Standard RNA extraction methods were used for the swab samples, a quick extraction method was used for saliva. Handling high-viscosity samples is discussed.
Read 20 tweets
Serve ancora fare chiarezza? A volte ho la sensazione che ciò che pare ovvio agli addetti ai lavori non lo sia altrettanto per chi non è solito usarle e non è stata fatta una buona campagna informativa. Metti, togli, serve, non serve.
Vediamo di chiarire. Image
La #mascherina serve soprattutto al chiuso, dove le #goccioline di saliva o respiratorie (#droplet) ristagnano o anche ora, in estate, sono trasportate dagli impianti di #ariacondizionata.
All’aperto bisogna stare molto vicini ad una persona infetta per essere contagiati. Image
Ma non tutte vanno bene. L’ideale sarebbe indossare mascherine #ffp3, che ci proteggono, ma voi capite che non bastano per tutti. Sono care. E comunque non proteggono gli altri, perché la valvola blocca in entrata ma non in uscita. Qui sopra uno non protegge Image
Read 8 tweets
Reporting progress on #coronavirus Home Test from our teams internal email: Just wanted to give a brief update from the #molbio team as we build momentum towards delivering an at-home, user-friendly, rapid #COVID19 test...We are actually detecting Covid19 #RNA in solution now! 1/
... we got early proof today that we can do reactions from RNA (the genetics code of #SARSーCoVー2 not DNA so it’s more insidious). Our method (which amplified the RNA without a machine) is very tolerant of #saliva - (we still have to crack open the virus RNA for full proof) 2/
The parenthesis in the above tweets are my commentary and I also edited out jargon. But wanted to share our internal optimism. We now know, except for breaking the #virus open to expose its #RNA we should be able to do a #COVID19 test without a #PCR machine @yale and @Penn 3/
Read 11 tweets

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