Discover and read the best of Twitter Threads about #crispr

Most recents (24)

10/2019: UK Genetics Lab Told To Hand Back DNA of African Tribes to Western Cape’s Stellenbosch Univ▫️

Whistleblowers, Formerly of Cambridge-based #WellcomeSanger Institute, Claimed Lab Wanted Use the #DNA to Make Money▫️

@POTUS
thetimes.co.uk/article/geneti…
dailymail.co.uk/sciencetech/ar…
“Welcomme Sanger has huge database of DNA in UK”

2020: “Launches Whole Genome Sequence Alliance to Map Spread of Coronavirus”
sanger.ac.uk/news/view/uk-l…
“Malaria Experimental Genetics lab course focused on #CRISPR-Cas9 experimental design + hands-on #transfection experience using varied approaches, practical exercises in #genotyping of modified #parasites & more” bit.ly/MEGen19 #Plasmodium
Read 6 tweets
We are now entering into an exponential phase of #COVID19 in Australia. It is time to seriously act. Besides the official recommendations from WHO, Department of health, the University, it is time to seriously prepare for the outbreak, in short be ready.
The number one recommendation is to of course wash our hands. this is extremely important. Besides going into details, there are series of general recommendations from the department of Health www1.health.gov.au/internet/main/…
Avoid public gathering such as going to conferences and seminar is important. Every hour counts and avoiding this are critical. So awaiting until Monday to ban those doesn't make a lot of sense in my view and will results in spreading #COVID19 more quickly abc.net.au/news/2020-03-1…
Read 9 tweets
Happy to share our latest work on In Vivo Systematic Mutagenesis of a Human Enhancer @AxelVisel @diane_dickel @LenPennacchio @LBNLBioSci @BerkeleyLab

cell.com/cell/fulltext/…

Thread👇 (1/12)
A major challenge in human genomics is how to systematically assess the phenotypic impact of enhancer variants identified in human genetics studies. Mouse transgenics is a powerful tool for this purpose but has limited throughput, high cost, and so-called position effects (2/12)
To overcome these limitations, we developed a high-throughput mouse enhancer-reporter assay (enSERT) that relies on #CRISPR/#Cas9-mediated site-specific integration of a transgene into a safe harbor location of the mouse genome and supports expression in all tissues (3/12)
Read 13 tweets
The #CRISPR human genome editing review I've been waiting for, from/by Jennifer @doudna_lab @nature nature.com/articles/s4158…
@doudna_lab @nature When you're really great in academics, you have 7 superscripts, and everybody wants to claim you :-)
@UCBerkeley
@berkeleyMCB
@QB3
@HHMINEWS
@igisci
@GladstoneInst
@LBNLBioSci
@UCB_Chemistry
@BerkeleyLab
@doudna_lab @nature @UCBerkeley @berkeleyMCB @QB3 @HHMINEWS @igisci @GladstoneInst @LBNLBioSci @UCB_Chemistry @BerkeleyLab "This profound opportunity to change #healthcare
for many people requires scientists, clinicians and bioethicists to work with healthcare economists and regulators to ensure safe, effective and affordable outcomes. The potential impact on patients
is too important to wait."
Read 3 tweets
#CRISPR #UniversalFluVaccine PART 1⃣ #AdaptiveImmuneSystem #TheSleeperHasAwakened Here are the "extras" in the vaccines. "Gene editing by exploiting the molecular components of the CRISPR bacterial adaptive immune system" sbgridconsortium.tumblr.com/post/183627174…
#CRISPRCas9 #UniversalFluVaccine PART 2⃣ #AdaptiveImmuneSystem #TheSleeperHasAwakened
CRISPR-Cas: microbial #AIS characterized by inserting short viral-or plasmid-derived DNA fragments (spacers) into the CRISPR regions in the microbe’s chromosome. nature.com/articles/s4159…
Read 3 tweets
Analysis: #NASDAQ $CRSP

Case 149 #CRISPR Therapeutics AG

DISCLAIMER: The analysis is strictly for educational purposes and should not be construed as an invitation to trade.

Thread 👇👇👇

#CRSP 1/4
Chart 1
Weekly Chart: The strong up move seen in Oct - Nov 2019 was rejected practically at the all time high at 73.90 just topping the level by 10 cents and the subsequent slide from there found #support just ahead of .....

CRSP 2/4
..... the Fib. 0.382.

The immediate trend remains #bearish - a close below 46.10 will immediately target 42.88/76 and below there is #trendline #support at 41.10.

CRSP 3/4
Read 4 tweets
"Prime" #CRISPR genome editing: a new chapter integrating reverse transcriptase for search and replace that is yet more versatile and precise; might be suitable for approaching 89% of disease-causing mutations
nature.com/articles/s4158…
@nature by David Lui and team @broadinstitute
@nature @broadinstitute 2 different ways to represent this significant advance 😉
1. for the genomics community
by @davidrliu @liugroup @broadinstitute
2. for the uninitiated
technologyreview.com/s/614599/the-n… by @antonioregalado @techreview
@nature @broadinstitute @davidrliu @liugroup @antonioregalado @techreview And a 3rd representation, explainer by @MeganMolteni and @WIREDScience wired.com/story/a-new-cr…
and a very good critique thread by @circuit_logic via @antonioregalado (+1, get rid of the 89%)
Read 3 tweets
Ein kleiner Exkurs ins #Gentechnik-Recht und warum derzeit so deutlich eine Änderung dieser Regelungen gefordert wird:
Grundlage für die nationalen Regelungne der EU-Mitgliedsstaaten ist die Freisetzungsrichtlinie 2001/18/EG vom 12.03.2001.
eur-lex.europa.eu/legal-content/…
Die GVO-Definition im Sinne der Richtlinie:
""genetisch veränderter Organismus (GVO)": ein Organismus mit Ausnahme des Menschen, dessen genetisches Material so verändert worden ist, wie es auf natürliche Weise durch Kreuzen und/oder natürliche Rekombination nicht möglich ist."
Zunächst fällt auf, dass von einem "genetisch veränderten" und nicht von einem "gentechnisch veränderten" Organismus die Rede ist. Deshalb braucht es auch den Nachsatz, dass die Veränderung nicht auf "natürliche Weise" möglich sein soll.
Read 13 tweets
97% of #cancer drugs tested fail. Why?
When @carlzimmer is on-target about off-target cancer drugs, as determined by #CRISPR @CSHL's @JSheltzer w/ a great team including undergrads, in @ScienceTM today
nytimes.com/2019/09/11/hea… @NYTScience
stm.sciencemag.org/content/11/509…
@carlzimmer @CSHL @JSheltzer @ScienceTM @NYTScience A succinct summary to this report @sciencemagazine by @jocelynkaiser science.sciencemag.org/content/365/64…
another way I can put it: Essential to acknowledge that the essentiality of genes many not be essential ;-)
Read 3 tweets
Ein unterbrechungsreicher Tag mit spontanem Besuch, Einkauf und reichlich Paketboten, an dem ich bisher nicht viel geschafft habe. Jetzt aber zum #Immunsystem! Grob gesagt besteht es aus einem stammesgeschichtlich alten, schnellen, unspezifischen Arm, der angeborenen Abwehr,
... oben im Bild, und einem erst bei der Entstehung der Wirbeltier aufgekommenen, langsameren, aber spezifischeren Arm, der adaptiven oder erworbenen Abwehr (unten). Das Ding mit der Pickelhaube ist ein Pathogen, die Wesen mit dem Kreis im Bauch (Zellkern) sind Immunzellen.
Die Zellen des Immunsystems arbeiten wie ein Fußballteam zusammen: In den vorderen beiden Reihen stehen die Zellen der angeborenen Abwehr (v. l. n. r.: Mastzellen, dendritische Zellen, Makrophagen, dahinter die Granulozyten), hinten die T- und B-Zellen der adaptiven Abwehr.
Read 27 tweets
New @biorxivpreprint from the group, setting our sites on Cas12a (the #CRISPR enzyme formerly known as Cpf1) as a simpler system for multiplexed genetic screens. Thread...

Optimization of AsCas12a for combinatorial genetic screens in human cells biorxiv.org/content/10.110…
@biorxivpreprint Cas12a has a major benefit, only needing one promoter for expressing multiple guides, and has a smaller constant region (the tracrRNA-like ‘handle’ for Cas12a, the DR, is only 20nts). So, a second guide is only 43 more nts -- much easier to write (synthesize) and read (sequence)
@biorxivpreprint Anyway, Cas12a has been around for a little while now, but not much uptake for screens. Part of that is likely because SpCas9 already has a lot of infrastructure around it, but probably also because there’s been less work on Cas12a to really bring it up to speed.
Read 14 tweets
Breaking↘️ @US_FDA Finds Unexpected Antibiotic Resistance #AMR #Genes in ‘Gene-Edited’ Dehorned #Cattle: "The @Recombinetics gene-edited cattle do contain #DNA unnatural to cattle". independentsciencenews.org/news/fda-finds… @Act4AnimalsEU
@US_FDA @Recombinetics @Act4AnimalsEU "FDA’s demonstration that gene-editing techniques can introduce foreign DNA is likely to be seen as a significant blow to the no-regulation argument. It is also a powerful vindication of the #EU approach, which is to regulate gene-edited organisms as #GMOs."
@US_FDA @Recombinetics @Act4AnimalsEU This is one more and very compelling argument to be critical of #CRISPR technologies to genetically modify animals. We recently expressed more criticism here, with regard to using gene editing to prevent puberty in #pigs: allianceforscience.cornell.edu/blog/2019/07/a…
Read 3 tweets
"Comment nourrir le monde sans détruire la planète?"

Joli challenge !

🇫🇷 translate.google.com/translate?hl=f…

🔽

How to feed the world without destroying the planet on.natgeo.com/2JKnRRl

En vérité, ceux qui me suivent en ont déjà entendu parler .../

⤵️
/...
Un pré-rapport avait été sorti en Décembre dernier, j'en avais fait un résumé :



Le rapport plus complet (564 pages) est disponible là :
sustainablefoodfuture.org/sites/default/…

Encore une fois, comme en Décembre, l'information ne sera probablement pas diffusée. 😔
Dans son interview, @waiterich insiste une nouvelle fois sur la nécessité d'augmenter la productivité de l'agriculture mondiale.

C'est la notion de land-Sparing : Produire sur le plus faible espace possible pour minimiser l'impact de l'agriculture sur la Biodiversité.

⤵️
Read 15 tweets
Et puisque ça parle de recherche génétique, regardons un peu ce qui se passe du côté de l'édition génétique à vocation agricole et alimentaire

C'est l'objet de cette publication :

…ntalevidencejournal.biomedcentral.com/track/pdf/10.1…

Quelques petits graphiques à extraire.

#CRISPR

⤵️
Tout d'abord, on voit que l'édition génétique est en train de bousculer la recherche sur l'agriculture.

Le nombre de publications prend une courbe exponentielle :

PS : 2018, ce sont des résultats partiels (5 mois)

⤵️
Qui publie sur l'édition génétique à vocation agricole?

2 pays se taillent la part du lion : la Chine et les USA bien sûr.

Quelques publications Françaises mais on est très loin de rivaliser.
Même les allemands publient 3 fois plus que nous :

⤵️
Read 11 tweets
In this article, we express our critical position towards using #CRISPR to engineer #pigs so that do not reach puberty. #Livestock farming should be designed with animal sentience at its core, we should not modify #animals to fit inhumane systems. allianceforscience.cornell.edu/blog/2019/07/a…
One aspect that the article does not mention and that we clearly indicated as being problematic is animal suffering during #experiments involving #CRISPR: painful #malformations, #abortions, are common "side effects" of such experiments.
Our original statement said "#CRISPR has a high potential for causing severe #animal #suffering during the research phases (such as abortions, perinatal mortality, malformations)
Read 6 tweets
Hoy voy a contar en un hilo mi charla del @pintofscienceES #Pint19TFE para aquellos q no pudieron ir
Se llama "Transgénicos naturales. No hemos inventado nada" donde repaso los casos de Transferencia horizontal de genes (THG) q he visto durante mientras investigaba en el lab
Y empiezo con esta imagen preguntando al público si creen q alguno de estos es un transgénico
Hay una encuesta en el siguiente tweet por si alguien quiere participar (las respuestas, al final del hilo)
¿Ves algún transgénico en esa imagen?
Read 43 tweets
Last day of #Microbio19 and #antibioticmakers session! Chair @kate_duncan and #ECMForum CoChair @Becca_McHugh introducing the morning session and first speaker
First speaker #antibioticsmakers #microbio19 is @NadineZiemert discussing her lab's work on novel tools and methods for #genomemining for prioritizing which biosynthetic gene clusters to follow up on for novel #bioactives #discovery
Nadine @NadineZiemert shows automated tools to find new antibiotic targets, and many other cool tools for screening genomes on antibiotic related info
Read 29 tweets
This @NatureMedicine paper shows 1 year after IM injection of #CRISPR AAV-SaCas9 for DMD:
-> editing event still occur 6 months after IM
-> immune response is seen within weeks & sustained
- on-target effects < 5% with AAV integration in genome

#paywall
nature.com/articles/s4159…
@NatureMedicine I have additional comments on this paper:

1/ it is great to see a year follow up after AAV injection. Important to see more of those.

2/ Immunogenicity of AAV and Cas9 are now well documented and this work confirm it. Interestingly Neonatal injection seems to overcome it
3/ Off target effects are minimal & again in line with previously reports (Table S1 & S2)

4/ Interestingly they found on-target effects with deletion, integration of AAV or increase circular RNA. Nextera technique is interesting. Would have loved to see some long read sequencing
Read 4 tweets
Today I received a parcel I wasn't expected from my friend @LluisMontoliu. I had no idea what it was and was intrigued to discover what it could be. So I opened it.
@LluisMontoliu What a wonderful surprise ! @LluisMontoliu 's book on #CRISPR ! I am so grateful to have received it from such a great friend and looking so much forward to read it (although I will need help from my wife for Spanish to French/English translation). Please buy the book !
Read 3 tweets
Les quiero contar algo sobre virus, arqueas, las archifamosas #CRISPR, Alicante y heces.

En 2015, investigadores estadounidenses analizaron las muestras de heces de personas americanas, francesas, gambianas y hasta de simios del zoo de San Francisco. Todas las muestras
procedían de individuos y animales que sufrieron brotes de diarrea típicos de una gastroenteritis viral.

Al determinar el #virioma en la muestras de heces (todos los virus presentes), notaron que una alta incidencia de un virus desconocido para la ciencia.
Lo llamaron smacovirus. Estos virus son realmente diminutos, su genoma es de apenas 2.5 Kb.

Para que tengan una idea, el genoma del VIH es de 9.2 Kb, el de zika de 11 Kb y el del ébola de 18 Kb, aproximadamente.

Sin embargo, no pudieron infectar ratones con #smacovirus.
Read 14 tweets
So the #CRISPR2019 conference program is up crispr2019.org & to my knowledge this is the first time in a #CRISPR conference there are more women invited speakers than men (13/21) crispr2019.org/invited-speake…. Kudos to the organizers @smoineau @theMaxwellLab et al...
Theoretically It shouldn't be too hard to organise a conference with 50/50 ratio men/women invited speakers. However for some reason the average ratio for a CRISPR conference is 20 to 30% and always the same persons are invited.
I have observed a very low diversity of talks + speakers on those conferences whereas there are tones of talented women and other people to those regularly invited working in the #CRISPR field
Read 4 tweets
This is a very interesting paper that came out in @CellCellPress on the generation of #CRISPR Cas9 variants by circular permutation (Cp-Cas9). Addition of a linker to a protease aka ProCas9 controls it activity to respond to viral infection cell.com/cell/fulltext/…
OK I have now a bit of time to elaborate more on this paper. What I like in this paper is the protein chemistry experiments displayed. I found the circular permutation with a peptide linker + screening technique really nice & very interesting to see CP on RuVC domain.
The replacement of the linker with a site specific protease sequence to render the Cas9 switchable is a really nice idea and clever.
Read 6 tweets
#365papers Let's try this for 2019, highlighting 365 papers (does not necessarily mean 1 paper per day) - A thread
#365papers 1⃣ The thread started with a bang - A must read opinion by @MarcHalfon on the fundamental question of how to define an enhancer cell.com/trends/genetic…
#365papers 2⃣ citrullination by PADI2 of RNA Pol II CTD R1810 regulates the transcription of highly expressed genes by mediating the interaction with P-TEFb cell.com/molecular-cell…
Read 367 tweets

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