Discover and read the best of Twitter Threads about #SARS_COV_2

Most recents (24)

Now that our SalivaDirect FDA EUA application for #SARS_CoV_2 diagnostics has been submitted, here is a sneak-peak at our validation results. medRxiv pre-print is forthcoming, but twitter is faster 😉

Overview: saliva w/o special tubes > no RNA extraction > dualplex PCR

About SalivaDirect ➡️…

Papers & Protocols ➡️…

SalivaDirect Results ➡️…

NBA validation study "Swish" ➡️
We validated SalivaDirect with multiple reagents & platforms to:
-make it available to more labs
-avoid specific supply chain bottlenecks
-keep costs down.

We'll provide specific information on how to conduct bridging studies to validate additional reagents/equipement
Read 10 tweets
Potser els virolegs ambientals (o jo) no hem explicat bé això de #coronavirus i temperatura. Són els virus sensibles a les temperatures d'estiu (i a la insolació i la radiació associada)? Sí. Són els #coronavirus en general i #SARSCoV2 sensibles a temperatures de 30-37ºC o més?
Taxativament sí, no els hi poso els articles; al meu TL els poden trobar. Però, és un procés q necessita el seu TEMPS (hores, dies) i afecta bàsicament a superfícies i fomites i també als exsudats....però les #fomites MAI van ser un vector principal de transmissió de #SARSCoV2...
i els exsudats, les microgotes, no tenen massa temps per patir les condicions ambientals si...som a 30 cm del company/a a la terrassa del bar, o dins; o a una barbacoa, o ens toquem, abracem, i ens exhalem l'un a l'altre...aquí no hi ha temperatures q hi jugui cap paper pq no...
Read 5 tweets
1/ Thread: Deeper dive into this fascinating autopsy series from the @davidadorward and colleagues with @COVID_ICECAP @EdinburghUni:

Tissue-Specific Tolerance in Fatal #Covid_19
- N= 11 patients
- Tissue Samples = 37 sites each (23 specific to respiratory tract) → 407 samples
- Mean Age: 77
- 10 (90.9%) patients male
- 4 (36.3%) patients received invasive mechanical ventilation

- #SARS_CoV_2 RNA was detected across ALL sampled organs and tissue sites
- Most frequent = Respiratory tract, GI tract, Heart, muscle
- Less frequent = Liver, Kidney
3/ Organ testing by several methods:

- Multiplex PCR mapped to SARS-CoV_2 genome
--> Presence of S protein evaluated on select PCR positive tissues
- Histological analysis of multiple tissues
Read 20 tweets
Nueva información que sugiere que el SARS-CoV-2 Sí se puede transmitir por el aire (y que la OMS ha actualizado hoy) como antes se creía improbable (como la OMS informaba hasta hoy). Abro hilo.
Igual cabe mencionar como introducción que la la OMS está "perdiendo credibilidad de le gente" por varios "cambios de opinión" a lo largo de la Pandemia como fueron las recomendaciones de EPP o el uso de Hidroxicloroquina, por ejemplo.
La respuesta que le daría a estas personas es que cuando se trata de algo nuevo, la credibilidad se basa en evidencias y estas a veces demoran en ser comprobadas, reproducibles y estandarizadas. Muchos conocimientos obvios de hoy quizá en un tiempo fueron poco claros antes.
Read 30 tweets
.@monaeltahawy has said, FUCK NORMAL, in a short video that rightly pins the pandemic on the status quo that made it possible. Going back to normal isn't what we need. 1/
My friend and colleague @akapczynski and I have talked about a new politics of care that is anything but normal, relies on overturning the political status quo I've lived with all my life. 2/…
I've been thinking about Virginia Woolf's "Jacob's Room" lately. It's a story about someone who is not coming back, a country where as Leonard Woolf said, "people are ghosts." 3/…
Read 21 tweets
Chlorhexidine (CHX) is very much effective against #SARS_CoV_2. The earlier notion that CHX may not work on the 'novel coronavirus' is outdated. Old studies had used very low conc. New evidence shows CHX effective against #2019ncov at 0.05% conc. #COVID19…
Study shows a single rinse with Chlorhexidine (CHX) mouthwash reduces SARS-COV-2 viral load in saliva for 2 hrs. Suggests CHX rinse to prevent #Covid19 spread via droplets.
Rinse 10 ml of 0.20% CHX for 30 sec


Full article:…
A single rinse with Chlorhexidine (CHX) mouthwash reduces SARS-COV-2 viral load in saliva for 2 hours. Rinse & gargle 10 ml of 0.20% CHX for 30 sec.
This evidence can be applied for prevention of #2019ncov spread in clinical & community settings. #COVID19
Read 4 tweets
@profchrisham @ONS There are 4 Pillars. Pillar 1 labs test the Swab by a technique called RT PCR, to see whether the patient has current infection with #SARS_CoV_2. This test is delivered by NHS and PHE labs. Pillar 2 labs do the same but are delivered by the Private Lighthouse labs. 1/n
@profchrisham @ONS 2/n The Pillar 1 labs declare their testing rate, & are a correct number of tests processed. Pillar 2 labs declare the tests processed AND the tests they have sent out. Test sent out should not be counted. Pillar 2 total tests are misleading.…
@profchrisham @ONS 3/n @profchrisham Pillar 3 testing is for antibody tests, to see whether you have had #SARS_CoV_2. These are mostly processed in NHS labs and the numbers are correct. But it is meaningless to add them to the number of RT PCR tests
Read 7 tweets
⚠️⚠️Communiqué de presse de la @SPILF_ et de toutes les instances de maladies infectieuses et tropicales représentant l'ensemble des médecins infectiologues français

"Une crise sanitaire ne justifie pas qu’on dise, ou qu’on fasse, n’importe quoi !"

Contact presse en DM
👇 1/n
Nos instances, représentant les médecins spécialisés en maladies infectieuses en France, souhaitent dénoncer les affirmations sans fondement qui ont été diffusées sur des chaînes de radio-télévision nationales au cours des derniers jours.

Elles sont d’autant plus graves qu’elles sont l’œuvre d’un médecin, chef de service dans un hôpital. Selon ses propos, 25 000 morts liées à la COVID-19 auraient pu être évitées en France si la combinaison hydroxychloroquine et azithromycine avait été prescrite massivement.

Read 17 tweets
Excited to announce our new study to develop and validate an inexpensive and sensitive saliva-based method for #SARS_CoV_2 testing in partnership with the @NBA.

Will be led by @awyllie13, @hmkyale, & myself from the @YaleSPH side.…
We'll be using our new #SalivaDirect protocol developed by @VogelsChantal + @DougBrackney. We'll have sensitivity data and a study design overview available on our website soon.…
Our study has 2 aims:

On the lab side, we are preparing #SalivaDirect for FDA EUA, and are validating with many different vendor reagents to drive down costs and not add supply chain bottlenecks. We are not seeking to commercialize any aspect. Our goal is to get this to $1/test
Read 9 tweets
🔥Voilà un thread afin de ne pas avoir à répéter tout le temps la même chose sur la recherche médicale thérapeutique pendant #COVID19 #SARS_CoV_2 #pandemic

1/ Les infectiologues cliniciens n'attendaient pas de ttt antiviral permettant une amélioration "spectaculaire" pr une maladie aiguë virale sévère (cf dengue, zika, chik, grippe), il était donc nécessaire d’évaluer rigoureusement et d’espérer un ttt un peu efficace et non toxique
2/ Il existe de nombreux candidats repositionnés qui ont été utilisés et testés et ce dès le début de la pandémie : #lopinavir/#ritonavir, #remdesivir, #hydroxychloroquine, #interferon ß pour n’en citer que quelques-uns
Read 35 tweets
New meta-analyisis estimates: (1) proportion of truly asymptomatic #COVID19 cases and (2) proportion of #SARS_CoV_2 transmission from asymptomatic or pre-symptomatic individuals [1/3]…
Overall, proportion of people who become infected with #SARS_CoV_2 and remain asymptomatic through the course of infection estimated to be 15% [2/3]
40-60% of #SARS_CoV_2 infections are the result of transmission from pre-symptomatic individuals. The contribution from asymptomatic individuals seems to be much smaller estimated at around 6% (but wide confidence intervals). [3/3]
Read 4 tweets
1. It's been frustrating to witness events unfold entirely as some predicted. My heart goes out to all those who've lost loved ones in the #COVID19 pandemic & those who are still suffering with illness.
2. In this March 2nd piece I warned a lethal pandemic was inevitable, the illness would not be mild, immunity would be uncertain, and if we didn't act quickly we'd be trapped with a high infection rate and a broken economy.…
3. We've made all the wrong choices and ended up exactly where I'd feared. So what now? It's clear some governments have given up. Lockdown is too hard. Test, track, isolate is too hard. The economic pain is too severe. They plan to let the virus run through their populations.
Read 22 tweets
1) Der super saubere #Sars_Cov_2-#PCR-Test von #Drosten, kann auf das Erkältungsvirus #Coronavirus #CoVOC43 kreuzreagieren. Die Möglichkeit von falsch-positiven Tests deshalb, ist wochenlang bekannt. #CoVOC43 kommt auch bei Tieren vor.

2) Gerade hier wird die ganze Glaubwürdigkeit von bestimmten Experten und sogenannten "#Faktenchecker|n" überdeutlich. Dass keine falsch-positiven Fälle bekannt sind, heisst nicht, dass es keine gibt, bei >95/98% Spezifität.…
3) Bei einer #Sars_CoV_2-Prävalenz(Infektionsdichte) von ca.1% o. darunter, wie zeitweise vermutet, ist das genauso, als wenn man mit einem guten Fernglas (PCR-Test) versucht, im dichten Nebel, scharfe Umrisse (Viren) zu erkennen. Es funktioniert nicht!
Read 6 tweets
'Rajasthan'....Why NOT getting some limelight in #COVID19 context? At a Glance:
- 5119/mil tests
- 66% recovery rate
- 31% active cases
- 2.23% mortality
- 3% growth rate, which needs to be down further.
The state is doing fine so far!! #rajasthancoronaupdate Image
States w/ 1000+ #Covid_19 cases: Sample tested until 31 May & Positive rate(%)
#Maharashtra 14.63%, highest
Trouble: Delhi, Telangana, Bihar & Gujarat, need intervention.
Excellent: Andhra, Karnataka, J& K positive rate below 1.5%.
Good: Rajashthan, Haryana, UP #COVID19India Image
As on 1 June, #TamilNadu added 1162 new cases. Positive rate stands at 9.77%, highest in last 7 days. Recovery 57%, Active cases 42%, mortality is just 0.79%. #TamilNaduCoronaupdate #IndiaFightsCoronavirus @Sheks65 @pibchennai
Read 18 tweets
THREAD: (1) A thought-provoking study by @Baric_Lab using reverse genetics and autopsy specimens demonstrates nasal ACE2 binding greater than in respiratory tract.…

#covid19 #coronavirus
(2) The nose contained highest percentage of ACE2 expressing cells. ACE2 levels waned in more distal bronchiolar & alveolar regions.
(3) ACE2 expression patterns were paralleled by high SARS-CoV-2 infectivity of nasal epithelium with marked reduction in distal lung (bronchioles & alveoli).
Read 13 tweets
1️⃣خلص كثير من الباحثين والمتخصصين في علم الفيروسات والأوبئة من خلال الدراسات الحديثة
التي أجريت خلال فاشية كورونا #SARS_CoV_2
إلى عدة استنتاجات و تصورات
يتبع ..
2️⃣ ينصح ان تتخذ الجهات الحكومية وغير الحكومية من هذه الاستنتاجات العلمية منصة لانطلاقتها في تنظيم اماكن و طريقة سير أعمالها في مرحلة تخفيف الحظر الكلي والعودة التدريجية للحياة العملية الجديدة
يتبع ..
3️⃣ ومما خلصوا إليه :

١ ) العوامل الرئيسية لإنتشار الفيروس

🛑التقارب لمدة طويلة (~ ١٥ دقيقة)
🛑البيئة المغلقة
🛑البيئة المكتظة المزدحمة
🛑البيئة الخالية من التهوية
🛑تجمعات الأصدقاء
🛑التجمعات العائلية

يتبع ..
Read 11 tweets
1/ THREAD: Memorial Day in a Pandemic

I often struggle with #MemorialDay. With the calls for celebration and happiness on this day. For BBQs and summer fun.

Because nothing about this day is happy.

This day is about #grief. About tragedy. About #death and loss.

🇺🇸 😔 😢
2/ Above all, it is a day of RESPECT. Because Americans gave their lives in service to protect the citizens and country of the United States.

The lives they previously dreamt of were cut short. And that selfless gift of life is one of the highest honors to serve your country. Image
3/ But their husbands and wives will never kiss their spouses again. Their children will never hug their mothers or fathers again. It doesn’t change the visceral grief of parents losing their children to #prematuredeath. #memorialdayweekend2020 Image
Read 31 tweets
Voilà qui nous obliger à changer notre façon de réfléchir le déconfinement. Science et The Atlantic se sont penchés sur la littérature récente concernant la propagation du #SARS_CoV_2 : la plupart des événements de contamination se font par voie aérosole en milieu fermé
En résumé, ce ne seraient pas les interactions secondaires (poignées de porte, bouton d'ascenseur etc) qui propageraient le virus, mais tousser, éternuer, postillonner, cracher etc. Exemple : le restaurant où une famille contaminée s'est assise sous l'air conditionné 3/n
Read 9 tweets
@SademiesO Pöhöttyneessä virkamieskunnassa on paljon mätää, joka imee kansantaloudesta elinvoimaa kuin 4. asteen syöpä.
- Tarvitsee vain puristaa kypsynyttä paisetta.
@SademiesO Sami Yläoutinen @SYlaoutinen VMn budjettipäällikkö.…

THL on löytänyt Suomesta vain 49 kpl hCoV -tapausta.
- Mutta #SARS_CoV_2 jota ei ole vielä löydetty Suomesta, on aiheuttanut seuraavaa:
@SademiesO @SYlaoutinen Marin on virkamieskätyreineen valmistellut Gatesin rahoittaman #WHO : n suunnitelmaa, ottaa Suomikin täyteen kontrolliin #CoronaVirusHOAX :n varjolla.
- Suunnitelma mureni Trump: in lopetettua rahoituksen ja Gates oikeuteen rikoksista ihmisyyttä vastaan.…
Read 8 tweets
1- Dün Lancet'de yayınlanan #COVID19 ilk insan Faz 1 çalışması ile ilgili yorumlarımı bugüne bıraktım. Çalışma ilk basılı Faz 1 sonuçları olması, Lancet'de yayınlanması anlamında önemli. Ama çalışmanın alt detaylarında devam eden süreçte yaşanabilecek zorluklar gözardı edilmemeli
2- Çalışma Wuhan'da yapılmış bir çalışma. Açık etiketli (yani herkes hangi dozda aşı olduğunu biliyor, aşı olan da uygulama yapılan da), randomize olmayan, 18-60 yaş arası erişkinlerde Mart ayında yapılan bir çalışma.
3- Çalışmada uygulanan aşı Ad5 vektör coronavirüs aşısı. Yapılan teknik daha öncede Oxford aşısında da bahsettiğim, coronavirüs spike proteinini vücuda girip koruyucu antikorlar üretmesi için bir başka virüs olan adenovirüse bağlıyorsunuz.
Read 21 tweets
Salah, bang Binsa. Herd immunity adalah keadaan di mana seseorang yg tak memiliki imunitas terlindungi dari infeksi bila 'kawanan' di mana ia berada mayoritas memiliki imunitas.

Imunitas tsb didapat dari vaksinasi atau sembuh dari infeksi, bukan sekedar daya tahan tubuh yg baik.
Permasalahan pd #COVID19, belum ada bukti bahwa sembuh dari infeksi virus #SARS_CoV_2 memberikan imunitas permanen.

Selain itu, imunitas yang sangat baik pada #COVID19 berpotensi memberikan masalah overimunitas, di mana pasien meninggal karena 'diserang' sistem imunnya sendiri
Tolong misinformasi ini dihapus, dibuat klarifikasi bahwa ini salah, dan dibuat komik strip yang benar. Terima kasih.

@TurnBackHoax @jokowi @TjahjantoHadi @Puspen_TNI @DivHumas_Polri @KemenkesRI @BNPB_Indonesia
#KomikKita #SiBinsa #P5TNI #HerdImmunity
Read 5 tweets
@THLorg ELISA -testit mittaavat IgG -vasta-aineet kaikille 6: lle (229E, OC43, SARS-CoV, NL63, HKU1, MERS-CoV) hCoV: lle. @SaneEpi @THLorg
- PCR RT -testi mittaa samoin vain proteiineja.

Millä testillä mittaatte #SARSCoV2 -viruksen, jota ei ole vielä löydetty ja eristetty?
@THLorg @SaneEpi Koronaepidemian väestöseerumi -tutkimuksen viikkoraportti päivitetty: 2020-05-20…
@THLorg @SaneEpi Suomen kattavissa THL seerumitutkimuksissa on löydetty 49 positiivista näytettä, ne kertovat 6: sta tunnetusta humanCoV: sta; (229E, OC43, SARS-CoV, NL63, HKU1, MERS-CoV) -#SARS_CoV_2 -positivisia ei ole löydetty.

Kuten ei 2m asukkaan Hampurissakaan
Read 5 tweets
Tweetorial: Just to let everyone know, I wear a mask in the OR for 10 hour cases, with EXTRA CO2 pumping into the sterile field to minimize intracardiac air. I have never once felt the need to take off my mask to breathe. (1/n)
Why do we pump CO2? If circulating room air gets in the heart, it’s 78% nitrogen, 21% oxygen, and relatively miniscule amounts of CO2. (2/n)
Oxygen dissolves in blood very well, but is flammable. Nitrogen is poorly soluble in blood. This is where “the bends” come from. (3/n)…
Read 9 tweets
"The live SARS-like coronavirus SL-CoV-WIV1 has been isolated for the first time from bat droppings; and such virus has been confirmed to invade the host cells through the ACE2 of human beings..." ImageImageImage
Bat Severe Acute Respiratory Syndrome-Like Coronavirus WIV1
Encodes an Extra Accessory Protein, ORFX, Involved in Modulation of the Host Immune Response. (2016) #ZhengLiShi #PeterDaszak #EcoHealth
Funding: #NIAID 110964 ImageImageImageImage
In this study, we have developed a fast and cost-effective method
for reverse genetics of coronaviruses by combining two approaches developed by others..As the genomes can be divided into multiple short fragments, mutations can be introduced into individual
fragments easily. ImageImageImageImage
Read 66 tweets

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